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Single-Molecule Studies of Nucleic Acids and Their Proteins [Kietas viršelis]

, (Research Director, LPS-ENS, PSL, CNRS, France), (Professor, LPS-ENS, PSL, CNRS, France), (Researcher, Department of Chemist), (Research Director and Professor, LPS-ENS, PSL, CNRS and Department of Chemistry and Biochemistry, UCLA, USA)
  • Formatas: Hardback, 240 pages, aukštis x plotis x storis: 249x178x17 mm, weight: 614 g, 41 grayscale and 45 color line figures; 8 grayscale and 9 color halftone figures
  • Serija: Oxford Graduate Texts
  • Išleidimo metai: 26-Dec-2018
  • Leidėjas: Oxford University Press
  • ISBN-10: 0198530927
  • ISBN-13: 9780198530923
Kitos knygos pagal šią temą:
Single-Molecule Studies of Nucleic Acids and Their ProteinsDidesnis paveikslėlis
  • Formatas: Hardback, 240 pages, aukštis x plotis x storis: 249x178x17 mm, weight: 614 g, 41 grayscale and 45 color line figures; 8 grayscale and 9 color halftone figures
  • Serija: Oxford Graduate Texts
  • Išleidimo metai: 26-Dec-2018
  • Leidėjas: Oxford University Press
  • ISBN-10: 0198530927
  • ISBN-13: 9780198530923
Kitos knygos pagal šią temą:
Pastovi nuoroda: https://www.kriso.lt/db/9780198530923.html
Raktažodžiai:
This book provides the basis for understanding the elastic properties of nucleic acids (DNA, RNA), the methods used to manipulate them (e.g. optical, magnetic and acoustic tweezers and traps), and how to observe their interactions with proteins (e.g. fluorescence microscopy, FCS, FRET, etc.). It then exemplifies the use of these various methods in the study of three families of DNA enzymes: polymerases, helicases and topoisomerases. The book aims not to be exhaustive, but rather to stimulate the imagination of readers in the application of these single molecule approaches to the study of DNA/RNA and their interactions.

Recenzijos

this book is helpful for different audiences... The volume is very well written and enjoyable to read. * Robert Ros, The Quarterly Review of Biology *

1 Introduction to DNA
1(10)
1.1 Introduction
1(1)
1.2 Single-molecule versus bulk studies
2(1)
1.3 DNA structures
3(8)
2 Manipulating DNA
11(16)
2.1 Tailoring and labelling DNA
11(1)
2.2 Molecular combing
12(3)
2.3 Forces at the molecular scale
15(1)
2.4 Micromanipulation tools
15(7)
2.5 Choosing a single-molecule manipulation tool
22(4)
2.6 Problems
26(1)
3 Fluorescence Spectroscopy and Microscopy Techniques
27(40)
3.1 Introduction
28(4)
3.2 Principles of fluorescence
32(17)
3.3 Illumination methods
49(6)
3.4 Detection techniques
55(3)
3.5 Single-molecule tracking
58(4)
3.6 Perspectives
62(1)
3.7 Problems
63(4)
4 The Mechanical Properties of Nucleic Acids
67(38)
4.1 Models of stretched polymers
69(5)
4.2 Relaxation dynamics of DNA
74(2)
4.3 The clastic behaviour of twisted DNA
76(6)
4.4 Torque estimate from single-molecule thermodynamics
82(4)
4.5 Twist-stretch coupling in DNA
86(2)
4.6 The braiding of two DNA molecules
88(2)
4.7 Simulations of stretched, twisted, or braided molecules
90(4)
4.8 Single-stranded nucleic acids
94(4)
4.9 Pulling on RNA
98(5)
4.10 Problems
103(2)
5 Structural Transitions in DNA
105(14)
5.1 Molecular transitions induced by twisting
105(5)
5.2 Molecular transitions induced by stretching
110(3)
5.3 Unzipping DNA
113(3)
5.4 Conclusions and perspectives
116(3)
6 Single-Molecule Studies of DNA-Associated Motors
119(2)
6.1 Introduction to DNA-protein interactions
119(2)
7 DNA and RNA Polymerases
121(34)
7.1 Introduction to polymerases
121(1)
7.2 An introduction to replication
121(2)
7.3 Single-molecule in vitro DNAP experiments
123(9)
7.4 In vivo studies of single replication complexes
132(1)
7.5 An introduction to transcription
133(6)
7.6 Single-molecule in vitro RNAP experiments
139(12)
7.7 In vivo studies of transcription
151(2)
7.8 Conclusions and perspectives
153(2)
8 DNA Helicases
155(22)
8.1 Introduction to helicases
155(3)
8.2 Bulk versus single-molecule assays: The case of RecBCD
158(2)
8.3 Active and passive unwinding mechanisms: RecQ versus gp41
160(4)
8.4 The UvrD helicase studied by single-molecule manipulations
164(7)
8.5 Single-molecule FRET studies of the Rep helicase
171(3)
8.6 Problems
174(3)
9 Topoisomerases
177(22)
9.1 Introduction to topoisomerases
177(3)
9.2 Bulk assays
180(1)
9.3 Single-molecule in vitro assays: Type I topoisomerases
181(4)
9.4 Single-molecule in vitro assays: Type II topoisomerases
185(10)
9.5 Single-molecule study of the coiling activity of gyrases
195(2)
9.6 In vivo studies of single topoIV localization and action in E.coli
197(2)
10 Conclusions
199(2)
References 201(22)
Index 223
David Bensimon and Vincent Croquette have been co-leaders of a group at the LPS-ENS that has been studying single DNA molecules and their interactions with proteins since 1991. Jean-Franēois Allemand, Terence Strick and Xavier Michalet have been their students and collaborators ever since. They have jointly developed magnetic traps and have been studying various aspects of single molecule DNA/protein transactions. Since joining the lab of Professor S. Weiss at UCLA , Xavier Michalet has been focusing on the use of fluorescent and spectroscopic methods to study single biomolecules.