El. knyga: SNAREs: Methods and Protocols

Molecular Dynamics Simulations of the SNARE Complex Interacting with Synaptotagmin, Complexin, and Lipid Bilayers.- Investigating complexin-membrane interactions using NMR and optical methods.- Molecular dynamics simulation for membrane fusion.- Use of Steered Molecular Dynamics to Explore the Conformational Changes of SNARE Proteins.- A Deep Learning and PSSM Profile Approach for Accurate SNARE Protein Prediction.- Use of Biotin-labeled Geranyl Pyrophosphate for Analysis of Ykt6 Geranylgeranylation.- Use of Bio-Layer Interferometry (BLI) to measure binding affinities of SNAREs and phosphoinositides.- Spectroscopic methods for detecting conformational changes during Sec18-Lipid interactions.- Assays of platelet SNARE-actin interactions.- Dissecting SNARE-Mediated Exocytosis in RBL-2H3 Mast Cells.- In vitro homotypic ER membrane fusion assay using isolated yeast microsomes.- Fluorescence anisotropy for monitoring cis- and trans-membrane interactions of synaptotagmin-1.- Structural Dynamics of SNARE Complex Assembly in the Ribbon Synapses Observed by smFRET.- FRAP assay to trace lipid mixing of the inner and outer leaflet of yeast vacuoles: Assessing the fusion state in live cells.- Mechanistic Insights into Synaptotagmin-1 Mediated Membrane Fusion and Interactions.- Negative staining electron microscopy of a highly flexible Sec1/Munc18 protein complex stabilized by glutaraldehyde crosslinking.- Electron tomography of organelles and vesicles in the investigation of SNARE function and localization.- A Dual-Color Fluorescence-based Ratiometric Assay to Measure Endocytic Trafficking of Surface Proteins.- SNARE-mediated membrane fusion probed using a synthetic organelle in the living bacterium.- Bimolecular Fluorescence Complementation (BiFC) Technique for exocytic proteins in murine hippocampal neurons.