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Techniques in Histopathology & Cytopathology [Minkštas viršelis]

  • Formatas: Paperback / softback, 208 pages, aukštis x plotis: 279x216 mm, weight: 450 g
  • Išleidimo metai: 30-Sep-2017
  • Leidėjas: Jaypee Brothers Medical Publishers
  • ISBN-10: 9352701097
  • ISBN-13: 9789352701094
Kitos knygos pagal šią temą:
  • Formatas: Paperback / softback, 208 pages, aukštis x plotis: 279x216 mm, weight: 450 g
  • Išleidimo metai: 30-Sep-2017
  • Leidėjas: Jaypee Brothers Medical Publishers
  • ISBN-10: 9352701097
  • ISBN-13: 9789352701094
Kitos knygos pagal šią temą:
This book is a practical guide to histopathological and cytopathological techniques for disease detection and diagnosis.





Divided into fifteen chapter, the text begins with an overview of cells and tissue, discussion on microscopy, and an introduction to the importance of histopathology.





The following sections cover different techniques, each describing basic theory, procedure, potential difficulties, and then concluding with important subjective and objective questions.





Recent developments in the field including immunochemistry, automation, and microarray, are also discussed.





Each technique is explained with the help of diagrams and figures to assist understanding.





Key points









Practical guide to histopathological and cytopathological techniques Presented in a step by step approach, with illustrative diagrams and figures Discusses recent advances and procedures Includes chapter on safety in the histopathology laboratory
Chapter 1 Cell and Tissues
1(16)
Cell
1(1)
Cell Division
2(3)
Cell Metabolism
5(1)
Tissues
6(5)
Liquid Connective Tissue
11(6)
Chapter 2 Microscopy
17(15)
History of Optical Microscope
17(1)
Basic Terminology in Microscopy
17(1)
Parts of Microscope
18(2)
Care of Microscope
20(1)
Types of Microscopy
21(1)
Bright Field Microscopy
21(1)
Dark Field Microscopy
22(2)
Phase Contrast Microscopy
24(1)
Fluorescence Microscopy
24(1)
Electron Microscopy
25(1)
Types of Electron Microscope
26(2)
Confocal Scanning Optical Microscopy
28(1)
Deconvolution Microscopy and Image Reconstruction
29(1)
Polarization Light Microscopy
29(3)
Chapter 3 Introduction and Importance of Histopathology
32(8)
Importance of Histopathology
32(1)
Decomposition and Putrefaction
32(1)
Various Steps of Histological Techniques
33(1)
Specimen Collection
33(1)
Logging of Specimen
34(2)
Tissue Marking and Orientation
36(1)
Methods of Examination of Tissues
37(1)
Special Instructions on Specimen Collection and Handling
37(1)
Duties and Responsibilities
38(2)
Chapter 4 Tissue Fixation
40(11)
Functions of fixatives
40(1)
Properties of Fixatives
40(1)
Types of Fixatives
41(1)
Properties and Action of Some Important Fixatives
42(6)
Fixation of Specific Substances
48
Artefacts
47(1)
Preservation and Storage of Tissues
48(1)
Points to be Remember During Fixation of Tissues
48(1)
Special Fixatives for Special Tissue Samples
48(3)
Chapter 5 Decalcification
51(6)
Properties of Decalcifying Agents
51(1)
Technique of Decalcification
51(1)
Tests for Completion of Decalcification
52(1)
Decalcifying Agents
53(1)
Treatment after Decalcification
54(1)
Surface Decalcification
54(1)
Factors Affecting the Rate of Decalcification
55(1)
Tips for Proper Decalcification
55(2)
Chapter 6 Tissue Processing
57(20)
Principle of Tissue Processing
57(1)
Selection and Labeling of Tissues
57(1)
Completion of Fixation before Processing
57(1)
Post-fixation Procedures
57(1)
Dehydration
58(1)
Clearing
59(2)
Impregnation and Infiltration
61(3)
Automatic Tissue Processing
64(2)
Manual Tissue Processing
66(4)
Paraffin Wax Embedding
70(1)
Storage of Paraffin Blocks
71(1)
Celloidin and Low Viscosity Nitrocellulose Embedding
72(1)
Gelatin Embedding
72(1)
Plastic Embedding
73(1)
Resin Embedding
73(1)
Double Embedding
74(1)
Re-embedding
74(3)
Chapter 7 Microtomy and Section Cutting
77(16)
Microtomes
77(2)
Microtome Knives
79(4)
Section Cutting
83(6)
Attaching the Sections to Slides
89(4)
Chapter 8 Frozen Sections and Cryostat
93(7)
Principle of Frozen Sections
93(1)
Theory of Freezing
93(1)
Cryoprotectants
94(1)
Cryogen selection
94(1)
Freezing Microtome
95(1)
Methods of Cutting Frozen Sections
95(5)
Chapter 9 Staining
100(29)
Principle of Staining
100(1)
Types of Dyes and Stains
101(1)
Classification of Dyes or Stains
102(1)
Types of Staining Processes
103(3)
Staining Procedures
106(1)
Hematoxylin Stains
107(2)
Counter Stains
109(1)
Standard Hematoxylin and Eosin Staining Method for Paraffin Sections
110(1)
Special Staining Methods
110(13)
Staining of Frozen Sections
123(2)
Precautions Taken during Staining
125(1)
Causes of Poor Staining
125(4)
Chapter 10 Mounting of Sections
129(6)
Characteristics of Mountant
129(1)
Types of Mountant
129(4)
Restaining
133(1)
Labeling and Storage of Slides
133(1)
Coverslips
133(1)
Ringing Media
133(2)
Chapter 11 Cytopathology
135(21)
Types of Exfoliation
135(1)
General Cytological Changes in Cells during Malignancy
135(1)
Cytology of Normal Genital Tract
136(1)
Methods of Specimen Collection and Submission
137(1)
Gynecological Sample Collection
138(2)
Non-gynecological sample collection
140(2)
Fine Needle Aspiration Cytology
142(2)
Preservation of cytological specimens
144(1)
Preparation of Smears
145(1)
Fixation and Fixatives
146(2)
Cytological Staining Techniques
148(2)
Special Stains
150(4)
Mounting of Smear of Cell Samples
154(2)
Chapter 12 Museum Techniques
156(4)
Functions of Pathology Museum
156(3)
Safety in the Museum
159(1)
Chapter 13 Safety in Histopathology Laboratory
160(5)
Staff Training
160(1)
Basic Organization and Common Sense
160(1)
Infectious Hazards
160(1)
Radiations Hazard
161(1)
Equipment Hazards
162(1)
Practical Safety in Routine Use
162(3)
Chapter 14 Advances in Histopathology and Cytopathology
165(11)
Microwave Irradiation of Primary Tissue Fixation
165(1)
Ultrasonic Decalcification
165(1)
Microwave-stimulated Tissue Processing
166(1)
Ultrasound-stimulated Tissue Processing
167(1)
Automatic Knife Sharpners
168(1)
Ultramicrotomy
168(1)
Automatic Staining Method
169(1)
Endoscopy
169(1)
Colposcopy
170(1)
Autoradiography
171(1)
Immunohistochemistry
172(1)
Tissue Microarrays
173(3)
Chapter 15 Solution and Reagents
176(3)
Important Definitions
176(1)
Preparation of Standard Solutions
176(3)
Bibliography 179(2)
Glossary 181(6)
Index 187
Sadhana Vishwakarma MSc PhD

Professor, Technocrats Institute of Technology, Bhopal, Madhya Pradesh, India