El. knyga: Thermogenic Fat: Methods and Protocols

Differentiation of Human Pluripotent Stem cells (hPSCs) into Brown-Like Adipocytes.- Culture and Differentiation of Primary Preadipocytes from Mouse Subcutaneous White Adipose Tissue.- Isolation and Differentiation of Adipocyte Precursors Derived from Neonatal Murine Brown Adipose Tissue.- Determination of Adipocyte Size and Number.- Measuring Mitochondrial Uncoupling in Mouse Primary Brown Adipocytes Differentiated ex vivo.- Assessment of Bioenergetics in BAT Mitochondria.- Use of Isothermal Microcalorimetry to Measure Cellular Heat Production in Thermogenic Adipocytes.- Measurement of Intracellular Temperature in Brown Adipocytes using a Cationic Fluorescent Polymeric Thermometer.- Indirect Calorimetry to Assess Energy Balance in Mice: Measurement and Data Analysis.- Thermogenic Phenotyping in Mice.- Calculating Diet-Induced Thermogenesis in Mice.- Assessment of Brown and Beige Adipose Tissue Activation in Mice using PET/CT Imaging.- Imaging Brown Adipose Tissue with TSPO PET Tracers in Preclinical Animal Studies.- Use of CIDEA Reporter Mouse Model for Screening Thermogenic Fat Activating Drugs.- AAV-Mediated Gene Delivery to Mouse Brown Adipose Tissue.- Subcutaneous Transplantation of White Adipose Tissue.- Brown Adipose Tissue Transplantation.- Isolation and Characterization of Brown Adipose Tissue T Cells.- Isolation of Adipose Tissue Extracellular Vesicles.- Isolation and Mass Spectrometry-Based Profiling of Major Lipids in Brown Adipose Tissue.- Methods for Single Cell Transcriptomic Analysis of Adipose Tissue.