This book addresses the literature gap by providing both theoretical foundations and practical insights into in vitro plant secondary metabolite production. It emphasizes the urgency to address the strategies and scientific methods for producing the secondary metabolites in the laboratory conditions. This book includes chapters on various in vitro strategies and laboratory methods, which are crucial for understanding and implementing techniques in this applied field. It equips the readers with the knowledge essential for contributions to pharmaceuticals, agriculture and various industries, thus filling a critical void currently present in the literature and practical applications in this field.
This book is structured to cater to a diverse audience, including graduate students and researchers. It is specifically tailored to address the key aspects of plant cell, tissue and organ culture, and aligns seamlessly with the curriculum requirements for the courses offered in plant biotechnology. The modules on cell cultures, secondary metabolite production, and metabolic engineering are also integral components for these courses.
Chapter
1. Introduction to Plant Secondary Metabolites.
Chapter
2. An Overview of In Vitro Secondary Metabolite Production.
Chapter
3. Laboratory Design and Functionality.
Chapter
4. Practical Plant Tissue Culture: Techniques and Protocols.
Chapter
5. Callus and Cell Suspension Cultures for Secondary Metabolite Production.
Chapter
6. Practical Protocols of Callus and Cell Suspension Cultures.
Chapter
7. Root Cultures and In Vitro Secondary Metabolite Production.
Chapter
8. Practical Techniques for Establishing Root Cultures.
Chapter
9. Shoot Cultures and In Vitro Secondary Metabolite Production.
Chapter
10. Practical Techniques for Shoot Cultures.
Chapter
11. Factors Impacting the Accumulation of Bioactive Compounds in Cultured Plant cells and Hairy roots.
Chapter
12. Metabolic Engineering for Plant Secondary Metabolites Production.
Chapter
13. Practical Approaches for Metabolic Engineering.
Chapter
14. In Vitro Strategies for Enhanced Secondary Metabolite Synthesis.
Chapter
15. Practical Techniques for Enhanced Secondary Metabolite Production In Vitro.
Chapter
16. Bioreactors.
Chapter
17. Practical protocol for Production of Secondary Metabolites using Bioreactors.
Chapter
18. In Vitro Production of Colors and Pigments.
Chapter
19. Production Of Carotenoid Pigments through Callus Culture.
Chapter
20. Elicitation.
Chapter
21. Practical Protocol for Biotic Elicitation.
Chapter
22. Biotransformation of Compounds Catalyzed by Plant Cells, Hairy Roots and Enzymes.
Chapter
23. Analytical Methods for Screening High Yielding In Vitro Cultures.
Chapter
24. Protocols for Analytical Techniques used in In Vitro Culture Systems.
Chapter
25. Commercial In Vitro Secondary Metabolite Production - Challenges And Opportunities.
Dr. Anuradha M, Principal and Director R&D at Padmashree Institute of Management and Sciences, Bangalore, India is deeply involved in fostering innovations and entrepreneurship. she has served as a Scientist at Dr. Reddys Research Foundation and SAMI Labs, playing a key role in setting up tissue culture labs in both industry and academia. Her expertise in plant biotechnology, plant tissue culture, nutrition, and nutraceuticals is reflected in her 50+ published articles, books, and granted patents.
Dr. S. Balasubramanya is currently working as a general manager at the Association of Biotechnology Led Enterprises (ABLE). He has obtained his doctorate degree in botany from Bangalore University, India. His notable achievements include holding several patents, including a US patent (no. 9565804) focusing on enhancing secondary metabolites and nutrients in plants. His research focus is on medicinal plants, plant biotechnology and the promotion of sustainable practices.
