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In Vitro and In Vivo Hemolysis: An Unresolved Dispute in Laboratory Medicine [Kietas viršelis]

  • Formatas: Hardback, 110 pages, aukštis x plotis: 240x170 mm, weight: 230 g, 10 Tables, black and white; 10 Illustrations, black and white
  • Serija: Patient Safety
  • Išleidimo metai: 16-Jul-2012
  • Leidėjas: De Gruyter
  • ISBN-10: 3110246139
  • ISBN-13: 9783110246131
Kitos knygos pagal šią temą:
  • Formatas: Hardback, 110 pages, aukštis x plotis: 240x170 mm, weight: 230 g, 10 Tables, black and white; 10 Illustrations, black and white
  • Serija: Patient Safety
  • Išleidimo metai: 16-Jul-2012
  • Leidėjas: De Gruyter
  • ISBN-10: 3110246139
  • ISBN-13: 9783110246131
Kitos knygos pagal šią temą:
Defined as red blood cell break down and the release of hemoglobin and intracellular contents into the plasma, hemolysis can seriously impact patient care as well as the laboratory's reputation through its affect on test results. Therefore, the European Preanalytical Scientific Committee, in collaboration with the International Federation of Clinical Chemistry Working Group on Patient Safety, have designed a questionnaire to collect data on prevalence and management of hemolytic specimens referred to the clinical laboratories for clinical chemistry testing. This book will help identify the areas where hemolysis occurs most frequently, which can, in turn, guide further analysis about why it is occurring. Once these elements are known, practices and procedures can be implemented to dramatically reduce hemolysis and avoid erroneous laboratory results affecting patient care and increasing laboratory costs.

"Giuseppe Lippi and Gianfranco Cervellin, Azienda Ospedaliero-Universitaria di Parma, Italy; Emmanuel J. Favaloro, Westmead Hospital, Australia; Mario Plebani, Universit? degli Studi di Padova, Padua, Italy."
Preface vii
1 Structure and function of red blood cells 1(2)
2 Red blood cell parameters 3(4)
2.1 Hematocrit
3(1)
2.2 Mean corpuscular volume
3(1)
2.3 Mean corpuscular hemoglobin
3(1)
2.4 Mean corpuscular hemoglobin concentration
4(1)
2.5 Red blood cell distribution width
4(3)
3 Definition of hemolysis 7(2)
4 Prevalence of hemolyzed specimens in clinical laboratories 9(6)
5 In vivo hemolysis 15(6)
5.1 Causes
15(3)
5.2 Clinical presentation
18(1)
5.3 Diagnosis of hemolytic anemia
19(2)
6 In vitro hemolysis 21(14)
6.1 Unsuitable procedures during blood collection
21(3)
6.2 Blood collection with various devices and needles
24(7)
6.3 Sample transportation and storage
31(2)
6.4 Centrifugation of the specimen
33(2)
7 How to distinguish in vivo versus in vitro hemolysis 35(4)
8 Effects of in vitro hemolysis on laboratory testing 39(16)
8.1 Interference in clinical chemistry testing
39(8)
8.2 Interference in hemostasis testing
47(2)
8.3 Interference in immunoassays and electrophoresis
49(3)
8.4 Interference in ABO type and antibody screen testing
52(3)
9 Spurious hemolysis and veterinary medicine 55(2)
9.1 Blood collection from small animals and pets
55(1)
9.2 Interference of hemolysis in veterinary laboratory medicine
55(2)
10 Detection of hemolyzed specimens 57(6)
11 Management of hemolyzed specimens 63(18)
11.1 Local evaluation of the bias
63(6)
11.2 Surveys and recommendations
69(1)
11.3 General indications for preventing hemolysis during blood collection
69(2)
11.4 Preventing hemolysis from intravenous catheters
71(1)
11.5 Reliable identification of hemolyzed specimens
72(1)
11.6 Management of test results on hemolyzed specimens
73(8)
12 The challenge of synthetic blood substitutes 81(8)
12.1 Individual studies evaluating the interference of blood substitutes
81(5)
12.2 Detection and quantification of blood substitutes
86(2)
12.3 Final remarks on blood substitutes
88(1)
References 89(12)
Index 101
Giuseppe Lippi and Gianfranco Cervellin, Azienda Ospedaliero-Universitaria di Parma, Italy; Emmanuel J. Favaloro, Westmead Hospital, Australia; Mario Plebani, Universitą degli Studi di Padova, Padua, Italy.