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El. knyga: Yeast Functional Genomics: Methods and Protocols

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  • Formatas: PDF+DRM
  • Serija: Methods in Molecular Biology 2477
  • Išleidimo metai: 06-May-2022
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071622575
Kitos knygos pagal šią temą:
Yeast Functional Genomics: Methods and Protocols Didesnis paveikslėlis
  • Formatas: PDF+DRM
  • Serija: Methods in Molecular Biology 2477
  • Išleidimo metai: 06-May-2022
  • Leidėjas: Springer-Verlag New York Inc.
  • Kalba: eng
  • ISBN-13: 9781071622575
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This second edition volume discusses the latest techniques and protocols used in the field that were not covered in the previous edition. The chapters in this book are organized into five parts. Part One looks at transcriptomic analyses and Part Two covers DNA replication and protein/DNA interactions. Part Three discusses translation dynamics, protein complexes, and proteomics. Part Four looks at genotypic screens and phenotypic profiling, and Part Five explores in silico integration of functional genomics data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and practical, Yeast Functional Genomics: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about the evolving field of yeast.

Chapters 1, 9, 16, 20, 22, 24, and 25 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. 











 
Single-Cell RNA-Sequencing in Yeast using the 10x Genomics Chromium
Device.- Genome-Wide Profiling of Transcription Initiation with
STRIPE-seq.- A Modified Crosslinking Analysis of cDNAs (CRAC) Protocol for
Detecting RNA-Protein Interactions and Transcription at Single Nucleotide
Resolution.- High-Resolution Deep Sequencing of Nascent Transcription in
Yeast with BioGRO-seq.- Direct Sequencing of RNA and RNA Modification
Identification using Nanopore.- UNAGI: Yeast Transcriptome Reconstruction and
Gene Discovery using Nanopore Sequencing.- Identification of Taxonomically
Restricted Transcripts from Illumina RNA Sequencing Data.- FORK-seq: Single
Molecule Profiling of DNA Replication.- CUT&RUN Profiling of the Budding
Yeast Epigenome.- ChIP-SICAP: A New Tool to Explore Gene-Regulatory Networks
in Candida albicans and Other Yeasts.- Purification of Ribosome-Nascent-Chain
Complex for Ribosome Profiling and Selective Ribosome Profiling.- Single-Step
Affinity Purification (ssAP) and Mass Spectrometry of Macromolecular
Complexes in the Yeast S. cerevisiae.- Composition and Dynamics of Protein
Complexes Measured by Quantitative Mass Spectrometry of Affinity Purified
Samples.- Deep Mutational Scanning of Protein-Protein Interaction Stability
between Partner Expressed from Their Endogenous Loci In Vivo.- Proteomic
Mapping by APEX2-Catalyzed Proximity Labeling in Saccharomyces cerevisiae
Semi-Permeabilized Cells.- Quantitative Proteomics in Yeast: From bSLIM and
Proteome Discoverer Outputs to Graphical Assessment of the Significance of
Protein Quantification Scores.- A Strong Cation Exchange Chromatography
Protocol for Examining N-Terminal Proteoforms.- RNA Interference (RNAi) as a
Tool for High-Resolution Phenotypic Screening of the Pathogenic Yeast Candida
glabrata.- High Throughput Gene Mutagenesis Screening using Base
Editing.- Saturated Transposon Analysis in Yeast (SATAY) for Deep Functional
Mapping of Yeast Genomes.- High-Throughput, High-Precision Colony Phenotyping
with Pyphe.- Bulk-Fitness Measurements using Barcode Sequencing Analysis in
Yeast.- Prediction of Gene and Genomic Regulation in Candida Species, using
the PathoYeastract Database: A Comparative Genomics Approach.- Enabling
Studies of Genome-Scale Regulatory Network Evolution in Large Phylogenies
with MRTLE.- Omics Analyses, How to Navigate through a Constant Data Deluge.
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