The Zebrafish: Biology presents laboratory protocols and reviews that focus on Danio rerio, the tropical, freshwater fish that has emerged as an extremely powerful molecular-genetic model system for analysis of vertebrate development. This volume describes the embryology of the zebrafish, presents methods for cell culture and for the study of gene expression and function in development, highlights cellular techniques for analysis of morphogenesis, and concludes with protocols for the study of and descriptions of mutations that perturb organogenesis. Accessible to novices and "old hands" alike, this volume summarizes all aspects of zebrafish cell and developmental biology.
Introduction: H.W. Detrich and L. Zon, Overview of the Zebrafish System.
D. Kane, Early Embryonic Development. Cull Culture and General Methods: D.
Barnes, Zebrafish Embryonal Cell Culture. B. Paw and L. Zon, Primary
Fibroblast Cell Culture. G. Corley-Smith, Production of Zebrafish
Androgenotes. Gene Expression and Function in Development: T. Jowett,
Analysis of Protein and Gene Expression. M. Hammerschmidt, P. Blader and U.
Str?hle, Strategies to Perturb Zebrafish Development. S. Ekker and T. Hyatt,
Vectors and Techniques for Ectopic Gene Expression in Zebrafish. H. Sive and
Y. Grinblat, Analysis of Zebrafish Development Using Explant Culture Assays.
Early Embryonic Development: M. Mullins, Embryonic Axis Formation. M. Cooper,
Confocal Microscopic Analysis of Morphogenetic Movements. L. Solnica-Krezel
and J. Topczewski, Cytoskeletal Dynamics of the Zebrafish Embryo. M. Chem and
H.W. Detrich, Kinesin-Like Microtubule Motors in Early Development.
Organogenesis: C. Moens, Techniques in Neural Development. J. Malicki,
Development of the Retina. S. Johnson and P. Bennet, Growth Control in the
Ontogenetic and Regenerating Zebrafish Fin. L. Zon, D. Stainier, And L.
Parker, Vascular and Blood Gene Expression. P. Jagadeeswar and J. Sheehan,
Analysis of Hemostasis in Zebrafish. M. Fishman and Serluca, Cell Lineage
Tracing in Heart Development. I. Dawid, Neurogenesis. Subject Index.
