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El. knyga: Zebrafish: Cellular and Developmental Biology, Part A

Volume editor (Professor of Biochemistry and Marine Biology at Northeastern University)
  • Formatas: PDF+DRM
  • Serija: Methods in Cell Biology
  • Išleidimo metai: 24-Dec-2010
  • Leidėjas: Academic Press Inc
  • Kalba: eng
  • ISBN-13: 9780123848932
Kitos knygos pagal šią temą:
Zebrafish: Cellular and Developmental Biology, Part ADidesnis paveikslėlis
  • Formatas: PDF+DRM
  • Serija: Methods in Cell Biology
  • Išleidimo metai: 24-Dec-2010
  • Leidėjas: Academic Press Inc
  • Kalba: eng
  • ISBN-13: 9780123848932
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This volume of Methods in Cell Biology, the first of 3 parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases.

* Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
* Summarizes the Zebrafish Genome Project

This volume of Methods in Cell Biology, the first of 3 parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases.

* Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
* Summarizes the Zebrafish Genome Project

Daugiau informacijos

A complete compendia of laboratory protocols and reviews on the use of the zebrafish as an animal model of research
Contributors xi
Preface xiii
I Cellular Biology
1(70)
1 Analyzing Retinal Axon Guidance in Zebrafish
1(27)
Fabienne E. Poulain
John A. Gaynes
Cornelia Stacher Horndli
Mei-Yee Law
Chi-Bin Chien
I Introduction
4(1)
II Visualizing Retinal Axons
5(8)
III Perturbing the Retinotectal System
13(8)
IV Future Directions
21(7)
References
22(6)
2 Imaging Blood Vessels in the Zebrafish
Makoto Kamei
Sumio Isogai
Weijun Pan
Brant M. Weinstein
I Introduction
28(1)
II Imaging Vascular Gene Expression
29(1)
III Non-vital Blood Vessel Imaging
30(9)
IV Vital Imaging of Blood Vessels
39(12)
V Conclusion
51(5)
References
52(4)
3 Medaka Haploid Embryonic Stem Cells
Yunhan Hong
I Introduction
56(2)
II Methods
58(5)
III Applications of Haploid ES Cells
63(4)
IV Summary
67(4)
References
68(3)
II Developmental and Neural Biology
71(252)
4 Neurogenesis
Prisca Chapouton
Leanne Godinho
I Introduction
74(1)
II Neurogenesis During Development and in the Adult Brain
75(6)
III Methods for Studying Neurogenesis in the Developing and Adult Brain
81(22)
IV Specific Protocols to Study Adult Neurogenesis
103(5)
V Conclusion
108(20)
References
108(20)
5 Studying Peripheral Sympathetic Nervous System Development and Neuroblastoma in Zebrafish
Rodney A. Stewart
Jeong-Soo Lee
Martina Lachnit
A. Thomas Look
John P. Kanki
Paul D. Henion
I Introduction
128(1)
II The Peripheral Autonomic Nervous System
128(6)
III The Zebrafish as a Model System for Studying PSNS Development
134(10)
IV Zebrafish as a Novel Model for Studying Neuroblastoma
144(2)
V Conclusion and Future Directions
146(1)
VI Acknowledgments
146(8)
References
147(7)
6 Analysis of the Retina in the Zebrafish Model
Andrei Avanesov
Jarema Malicki
I Introduction
154(1)
II Development of the Zebrafish Retina
155(7)
III Analysis of Wild-Type and Mutant Visual System
162(20)
IV Analysis of Gene Function in the Zebrafish Retina
182(9)
V Summary
191(15)
References
192(14)
7 Photoreceptor Structure and Development: Analyses using GFP Transgenes
Brian D. Perkins
James M. Fadool
I Introduction
206(3)
II Transport Mechanisms
209(1)
III Regulation of Photoreceptor Size
210(1)
IV Photoreceptor Synapse Structure
211(1)
V Regeneration
211(4)
VI Future Directions
215(1)
VII Conclusions
216(4)
References
216(4)
8 Physiological Recordings from Zebrafish Lateral-Line Hair Cells and Afferent Neurons
Josef G. Trapani
Teresa Nicolson
I Introduction
220(1)
II Zebrafish Mounting and Immobilizing
221(2)
III Microphonics
223(2)
IV Action Currents
225(3)
V Summary
228(1)
VI Discussion
228(6)
References
229(5)
9 Zebrafish Kidney Development
Iain A. Drummond
Alan J. Davidson
I Introduction
234(2)
II Structure of the Zebrafish Pronephros
236(2)
III Formation of the Pronephros
238(7)
IV Methods to Study Pronephros Function
245(11)
V Conclusions
256(6)
References
256(6)
10 Molecular Regulation of Pancreas Development in Zebrafish
Robin A. Kimmel
Dirk Meyer
I Introduction
262(1)
II Pancreas Development
262(6)
III Analysis of Beta-Cell Migration and Proliferation
268(8)
IV Future Directions
276(6)
References
276(6)
11 Monitoring Sleep and Arousal in Zebrafish
Jason Rihel
David A. Prober
Alexander F. Schier
I Introduction
282(1)
II Behavior, Genetics, and Pharmacology of Zebrafish Sleep
282(6)
III Methods for Monitoring Sleep/Wake Behavior in Zebrafish
288(3)
IV Conclusion
291(5)
References
291(5)
12 Use of Flatbed Transparency Scanners in Zebrafish Research: Versatile and Economical Adjuncts to Traditional Imaging Tools for the Danio rerio Laboratory
Charles A. Lessman
Michael R. Taylor
Wilda Orisme
Ethan A. Carver
I Introduction
296(1)
II Scanner Basics
297(5)
III Motility Analysis
302(7)
IV Oocyte and Egg Assays
309(7)
V Using Scanners to Count and Measure
316(3)
VI Other Potential Applications of Scanners
319(2)
VII Summary: Inexpensive Adjunct to Microscopy
321(2)
References
321(2)
Subject Index 323(16)
Volumes in Series 339
Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.
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