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El. knyga: Zebrafish: Cellular and Developmental Biology, Part B

Volume editor H. William Detrich III (Professor of Biochemistry and Marine Biology at Northeastern University), Volume editor Leonard I. Zon (Boston Children's Hospital / HHMI, Boston, MA, USA), Volume editor Monte Westerfield (Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA)

Formatas: EPUB+DRM
Serija: Methods in Cell Biology
Išleidimo metai: 02-Jun-2011
Leidėjas: Academic Press Inc
Kalba: eng
ISBN-13: 9780123870377

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Formatas: EPUB+DRM
Serija: Methods in Cell Biology
Išleidimo metai: 02-Jun-2011
Leidėjas: Academic Press Inc
Kalba: eng
ISBN-13: 9780123870377

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This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendium of laboratory protocols and reviews covering all the new methods developed since 1999.

* Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
* Illustrates many techiques in full-color
* Summarizes the Zebrafish Genome Project

This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendium of laboratory protocols and reviews covering all the new methods developed since 1999.

* Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
* Illustrates many techiques in full-color
* Summarizes the Zebrafish Genome Project

Daugiau informacijos

Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls

Contributors

Preface

xiii

Part I Cell Biology

1 Live Imaging of the Cytoskeleton in Early Cleavage-Stage Zebrafish Embryos

(18)

M. Wuhr

N. D. Obholzer

S. G. Megason

H. W. Detrich III

T. J. Mitchison

I Introduction

(2)

II Maintaining the Breeding Competence of Zebrafish throughout the Day

(1)

III Mounting Zebrafish Embryos for Live Imaging

(1)

IV Live Imaging of Microtubules in Cleaving Zebrafish Embryos

(4)

V Live Imaging of Microfilaments in Cleaving Zebrafish Embryos

(2)

VI Comparison of Microscopic Techniques for Imaging the Cytoskeleton of Cleaving Zebrafish Embryos

(2)

VII Discussion and Future Directions

(5)

References

(4)

2 Analysis of Cell Proliferation, Senescence, and Cell Death in Zebrafish Embryos

(20)

Daniel Verduzco

James F. Amatruda

I Introduction: The Cell Cycle in Zebrafish

(2)

II Zebrafish Embryo Cell-Cycle Protocols

(9)

III Screening for Chemical Suppressors of Zebrafish Cell-Cycle Mutants

(3)

IV Conclusions

(1)

V Reagents and Supplies

(5)

References

(4)

3 Analysis of Cilia Structure and Function in Zebrafish

(36)

Jarema Malicki

Andrei Avanesov

Jade Li

Shiaulou Yuan

Zhaoxia Sun

I Introduction

(1)

II Cilia in Zebrafish Embryos and Larvae

(6)

III Analytical Tools for Cilia Morphology and Motility

(7)

IV Analysis of Cilia-related Mutant Phenotypes in Zebrafish

(14)

V Future Directions

(7)

References

(6)

4 Cellular Dissection of Zebrafish Hematopoiesis

(36)

David L. Stachura

David Traver

I Introduction

(1)

II Zebrafish Hematopoiesis

(10)

III Hematopoietic Cell Transplantation

(8)

IV Enrichment of Hematopoietic Stem Cells

(3)

V In vitro Culture and Differentiation of Hematopoietic Progenitors

(8)

VI Conclusions

105

(6)

References

105

(6)

5 Zebrafish Lipid Metabolism: From Mediating Early Patterning to the Metabolism of Dietary Fat and Cholesterol

111

(32)

Jennifer L. Anderson

Juliana D. Carten

Steven A. Farber

I Introduction

112

(2)

II Lipid Metabolism in Developing Zebrafish

114

(1)

III Yolk Metabolism During Early Vertebrate Development

115

(3)

IV Lipid Signaling During Early Zebrafish Development

118

(4)

V Visualizing Lipid Metabolism in Larval and Adult Zebrafish

122

(9)

VI Triple Screen: Phospholipase, Protease and Swallowing Function Assays

131

(3)

VII Zebrafish Models of Human Dyslipidemias

134

(1)

VIII Summary

135

(8)

References

136

(7)

Part II Developmental and Neural Biology

6 Development of the Zebrafish Enteric Nervous System

143

(18)

Iain Shepherd

Judith Eisen

I Introduction

144

(1)

II Organization of the Zebrafish Intestinal Tract

144

(2)

III Early Development of the ENS

146

(2)

IV Genetic Approaches to Studying ENS Development

148

(1)

V Molecular Mechanisms of ENS Development

149

(1)

VI ENS Differentiation

150

(1)

VII Regulation of Gut Motility

151

(2)

VIII Zebrafish ENS as a Model for Understanding Human Diseases

153

(2)

IX Future Prospects

155

(6)

References

156

(5)

7 A Guide to Analysis of Cardiac Phenotypes in the Zebrafish Embryo

161

(20)

Grant I. Miura

Deborah Yelon

I Introduction

161

(2)

II Defects in Heart Size

163

(5)

III Defects in Heart Shape

168

(6)

IV Defects in Cardiac Function

174

(2)

V Summary

176

(5)

References

176

(5)

8 Chemical Approaches to Angiogenesis in Development and Regeneration

181

(16)

Scan Hasso

Joanne Chan

I Introduction

182

(1)

II Chemical Approaches and Zebrafish Vascular Development

183

(6)

III Novel Chemical Strategies to Investigate Mechanisms of Angiogenesis

189

(3)

IV Summary

192

(5)

References

192

(5)

9 Laser-Induced Thrombosis in Zebrafish

197

(8)

Pudur Jagadeeswaran

Maira Carrillo

Uvaraj P. Radhakrishnan

Surendra K. Rajpurohit

Seongcheol Kim

I Introduction

197

(2)

II Vascular Occlusion

199

(1)

III Methods

199

(3)

IV Future Perspectives

202

(3)

References

203

(2)

10 Endoderm Specification, Liver Development, and Regeneration

205

(20)

Trista E. North

Wolfram Goessling

I Review of the Literature

206

(4)

II Embryonic and Larval Protocols to Analyze Liver Formation

210

(3)

III Liver Injury and Regeneration Protocols

213

(4)

IV Assessment of Liver Function

217

(2)

V Summary

219

(6)

References

219

(6)

11 Morphogenesis of the Zebrafish Jaw: Development Beyond the Embryo

225

(24)

Kevin J. Parsons

Viktoria Andreeva

W. James Cooper

Pamela C. Yelick

R. Craig Albertson

I Postembryonic Development - Framing the Questions and Understanding the Challenges

226

(2)

II Obtaining Phenotypes

228

(3)

III Quantitative Methods for Studying Adult Phenotypes

231

(11)

IV A Complementary Approach: The Use of Natural Variation to Complement that Generated in the Lab for Understanding Jaw Morphogenesis

242

(2)

V Implications and Conclusions

244

(5)

References

245

(4)

12 Associative Learning in Zebrafish (Danio rerio)

249

(22)

Robert Gerlai

I Introduction

250

(1)

II Rationale

251

(2)

III Methods and Discussion

253

(15)

IV Summary

268

(3)

References

268

(3)

Index

271

(12)

Volumes in Series

283

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms. Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA Grousbeck Professor of Pediatrics, Boston Children's Hospital / HHMI, Boston, MA, USA

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