Contributors |
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xiii | |
Preface |
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xvii | |
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Chapter 1 Methods to Study Maternal Regulation of Germ Cell Specification in Zebrafish |
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1 | (32) |
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2 | (1) |
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1 Stages of Maternal Germ Plasm Regulation and Specification |
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3 | (3) |
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2 Studying the Dynamic Localization of Germ Plasm RNAs |
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6 | (6) |
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3 Studying Molecular Requirements Through Loss-of-Function Approaches |
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12 | (5) |
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4 Utility of Transgenic Fish to Study Germ Cell Development |
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17 | (16) |
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23 | (1) |
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24 | (9) |
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Chapter 2 Patterning, Morphogenesis, and Neurogenesis of Zebrafish Cranial Sensory Placodes |
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33 | (36) |
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34 | (2) |
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1 Specification of the PPR and Patterning the PPR into Specific Placodes |
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36 | (11) |
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2 Cranial Placode Morphogenesis and Neurogenesis |
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47 | (22) |
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53 | (1) |
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54 | (1) |
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54 | (15) |
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Chapter 3 Oligodendrocyte Differentiation |
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69 | (28) |
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70 | (2) |
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1 Zebrafish As a Model to Study Oligodendrocyte Differentation |
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72 | (2) |
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2 Insights Into Oligodendrocyte Development and Myelination Using Zebrafish |
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74 | (12) |
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3 Zebrafish As Model to Study Remyelination |
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86 | (11) |
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Future Directions and Conclusions |
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87 | (1) |
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88 | (9) |
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Chapter 4 Studying the Peripheral Sympathetic Nervous System and Neuroblastoma in Zebrafish |
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97 | (42) |
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98 | (1) |
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1 The Peripheral Autonomic Nervous System |
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99 | (8) |
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2 The Zebrafish as a Model System for Studying PSNS Development |
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107 | (13) |
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3 Zebrafish as a Novel Model for Studying Neuroblastoma |
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120 | (19) |
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127 | (1) |
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127 | (1) |
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127 | (12) |
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Chapter 5 Zebrafish As a Model for Understanding Enteric Nervous System Interactions in the Developing Intestinal Tract |
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139 | (26) |
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140 | (3) |
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1 Genetic and Environmental Interactions During Enteric Nervous System Development |
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143 | (3) |
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2 Interactions Between the Enteric Nervous System and the Immune System |
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146 | (9) |
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3 Interactions Between the Enteric Nervous System and Effector Cells |
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155 | (2) |
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157 | (8) |
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157 | (1) |
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158 | (7) |
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Chapter 6 Methods to Study the Development, Anatomy, and Function of the Zebrafish Inner Ear Across the Life Course |
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165 | (46) |
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166 | (3) |
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1 Imaging Methods for Analysis of the Zebrafish Ear and Lateral Line |
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169 | (13) |
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2 Experimental Manipulation of Otic Development |
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182 | (4) |
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3 Dissection and Imaging of the Adult Ear and Otoliths |
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186 | (3) |
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189 | (6) |
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5 Small Molecule Screening |
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195 | (2) |
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6 Zebrafish Models of Human Deafness and Vestibular Disorders |
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197 | (2) |
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199 | (12) |
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200 | (1) |
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200 | (11) |
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Chapter 7 Imaging Collective Cell Migration and Hair Cell Regeneration in the Sensory Lateral Line |
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211 | (46) |
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213 | (1) |
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1 Lateral Line Development |
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213 | (3) |
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2 In Vivo Imaging of Lateral Line Primordium Migration |
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216 | (12) |
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3 Live Labeling of Lateral Line Cells |
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228 | (8) |
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4 Nonvital Tissue Labeling of Lateral Line Cells |
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236 | (2) |
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5 Interpretation of Common Phenotypes |
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238 | (1) |
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239 | (1) |
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7 Long-Term Time-Lapse Analyses of Regenerating Neuromasts |
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239 | (18) |
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248 | (1) |
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248 | (1) |
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249 | (8) |
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Chapter 8 Analysis of the Retina in the Zebrafish Model |
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257 | (78) |
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258 | (2) |
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1 Development of the Zebrafish Retina |
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260 | (7) |
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2 Analysis of the Visual System in Wild Type and Mutants |
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267 | (33) |
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3 Analysis of Gene Function in the Zebrafish Retina |
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300 | (35) |
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310 | (1) |
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311 | (1) |
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311 | (24) |
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Chapter 9 Strategies for Analyzing Cardiac Phenotypes in the Zebrafish Embryo |
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335 | (34) |
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335 | (3) |
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1 Regulation of Heart Size |
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338 | (7) |
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2 Regulation of Cardiac Morphology |
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345 | (10) |
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3 Regulation of Cardiac Function |
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355 | (5) |
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360 | (9) |
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360 | (9) |
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Chapter 10 Chemical Approaches to Angiogenesis in Development and Regeneration |
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369 | (8) |
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370 | (1) |
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1 High-Throughput Screening for Small Molecules With Proangiogenic Activity Using Primary Cell Culture of Tg[ flk: GFP] Zebrafish Embryos |
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370 | (3) |
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2 Validation of Proangiogenic Compounds in Vivo Using Pre-inhibited Vascular Structure of Zebrafish Embryo by VRI |
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373 | (1) |
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373 | (1) |
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374 | (3) |
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375 | (1) |
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376 | (1) |
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Chapter 11 Quantitative Methods for Studying Hemostasis in Zebrafish Larvae |
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377 | (14) |
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378 | (1) |
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379 | (12) |
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386 | (2) |
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388 | (1) |
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388 | (3) |
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Chapter 12 Zebrafish Kidney Development |
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391 | (40) |
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392 | (1) |
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1 Structure of the Zebrafish Pronephros |
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393 | (4) |
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2 Formation of the Pronephros |
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397 | (9) |
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3 Methods to Study Pronephros Function |
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406 | (25) |
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422 | (1) |
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422 | (1) |
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423 | (8) |
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Chapter 13 Zebrafish Pancreas As a Model for Development and Disease |
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431 | (32) |
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432 | (1) |
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1 Molecular Mechanisms of Secondary Islet Formation |
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433 | (5) |
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2 Physiology and Assessment of Glucose Homeostasis in Zebrafish |
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438 | (3) |
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3 Diabetes and Related Disease Models in Zebrafish |
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441 | (5) |
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4 Methods to Study Beta Cell Biology and Physiology |
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446 | (10) |
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456 | (7) |
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457 | (1) |
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457 | (6) |
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Chapter 14 Endoderm Specification and Liver Development |
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463 | (22) |
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464 | (1) |
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1 Review of the Literature |
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464 | (6) |
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2 Embryonic and Larval Protocols to Analyze Liver Formation |
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470 | (2) |
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3 Liver Injury and Regeneration Protocols |
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472 | (13) |
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476 | (1) |
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476 | (9) |
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Chapter 15 Emerging Tools to Study Proteoglycan Function During Skeletal Development |
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485 | (46) |
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486 | (10) |
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1 Xylose: Where the "Proteo-" meets the "-Glycan" |
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496 | (3) |
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2 Adding Sugar Like a Kid After Halloween |
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499 | (1) |
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3 Don't Be a Quitter: Posttranslational Modifications of Posttranslational Modifications |
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500 | (1) |
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4 Break it Down for Me, Fellas |
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501 | (2) |
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5 Adding Function to Structure by Understanding PG-Loss Animal Models |
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503 | (6) |
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6 Heres Looking at You, PG |
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509 | (22) |
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513 | (1) |
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514 | (1) |
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514 | (17) |
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Chapter 16 Generation and Analysis of Zebrafish Melanoma Models |
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531 | (20) |
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532 | (1) |
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1 Genetic Models of Melanoma in Zebrafish |
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532 | (5) |
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2 Cell Line Models of Melanoma in Zebrafish |
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537 | (6) |
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543 | (3) |
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546 | (5) |
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546 | (1) |
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547 | (4) |
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Chapter 17 Learning and Memory in Zebrafish (Danio rerio) |
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551 | (36) |
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552 | (29) |
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581 | (1) |
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581 | (1) |
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581 | (6) |
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Chapter 18 Working With Zebrafish at Postembryonic Stages |
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587 | (22) |
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588 | (2) |
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1 Part I: Postembryonic Staging |
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590 | (8) |
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2 Part II: Rearing Fish for Use at Postembryonic Stages |
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598 | (4) |
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602 | (7) |
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603 | (1) |
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603 | (6) |
Volumes in Series |
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609 | (14) |
Index |
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623 | |