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Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology 4th edition, Volume 133 [Kietas viršelis]

Volume editor (Professor of Biochemistry and Marine Biology at Northeastern University), Volume editor (Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA), Volume editor (Boston Children's Hospital / HHMI, Boston, MA, USA)
  • Formatas: Hardback, 322 pages, aukštis x plotis: 235x191 mm, weight: 930 g
  • Serija: Methods in Cell Biology
  • Išleidimo metai: 13-Jun-2016
  • Leidėjas: Academic Press Inc
  • ISBN-10: 0128034750
  • ISBN-13: 9780128034750
Kitos knygos pagal šią temą:
  • Formatas: Hardback, 322 pages, aukštis x plotis: 235x191 mm, weight: 930 g
  • Serija: Methods in Cell Biology
  • Išleidimo metai: 13-Jun-2016
  • Leidėjas: Academic Press Inc
  • ISBN-10: 0128034750
  • ISBN-13: 9780128034750
Kitos knygos pagal šią temą:

The Zebrafish: Cellular and Developmental Biology Part A, Fourth Edition, is the latest edition in the Methods in Cell Biology series that looks at methods for analyzing cellular and developmental biology of zebrafish. Chapters cover such topics as cell biology and developmental and neural biology.

  • Covers sections on model systems and functional studies, imaging-based approaches, and emerging studies
  • Written by experts in the field
  • Contains cutting-edge material on the topic of developmental biology in zebrafish
  • New two part edition of this important volume

Recenzijos

Praise for the Series: "The series is invaluable for workers at all levels of cell biology." --Nature

Daugiau informacijos

This new Fourth edition of Methods in Cell Biology looks at methods for cellular and developmental biology in zebrafish
Contributors xi
Preface xv
Chapter 1 Embryonic Cell Culture in Zebrafish
1(10)
C.A. Ciarlo
L.I. Zon
Introduction
2(1)
1 Methods
3(8)
Conclusion
10(1)
References
10(1)
Chapter 2 Cellular Dissection of Zebrafish Hematopoiesis
11(44)
D.L. Stachura
D. Traver
Introduction
12(1)
1 Zebrafish Hematopoiesis
12(12)
2 Hematopoietic Cell Transplantation
24(8)
3 Enrichment of HSCs
32(3)
4 In Vitro Culture and Differentiation of Hematopoietic Progenitors
35(20)
Conclusions
46(1)
References
46(9)
Chapter 3 Second Harmonic Generation Microscopy in Zebrafish
55(14)
D.C. LeBert
J.M. Squirrell
A. Huttenlocher
K.W. Eliceiri
Introduction
56(3)
1 Materials
59(3)
2 Methods
62(4)
3 Notes
66(3)
Acknowledgments
66(1)
Supplementary Data
67(1)
References
67(2)
Chapter 4 Imaging Blood Vessels and Lymphatic Vessels in the Zebrafish
69(36)
H.M. Jung
S. Isogai
M. Kamei
D. Castranova
A.V. Gore
B.M. Weinstein
Introduction
70(1)
1 Imaging Vascular Gene Expression
71(3)
2 Nonvital Blood Vessel and Lymphatic Vessel Imaging
74(9)
3 Vital Imaging of Blood and Lymphatic Vessels
83(22)
Conclusion
98(1)
References
99(6)
Chapter 5 An Eye on Light-sheet Microscopy
105(20)
D. Kromm
T. Thumberger
J. Wittbrodt
Introduction
106(1)
History
106(1)
1 Principle Behind Selective Plane Illumination Microscopy
107(7)
2 The Microscope for Your Sample or the Sample for Your Microscope?
114(3)
3 Data Acquisition and Handling
117(2)
4 Challenges and Perspectives
119(6)
Acknowledgment
120(1)
References
121(4)
Chapter 6 Single Neuron Morphology In Vivo with Confined Primed Conversion
125(14)
M.A. Mohr
P. Pantazis
Introduction
126(1)
1 Photoconvertible Fluorescent Proteins
127(1)
2 Confined Primed Conversion
128(1)
3 Unraveling Single Neuron Morphology With Confined Primed Conversion
129(10)
Conclusion and Outlook
135(1)
References
136(3)
Chapter 7 Visualizing Retinoic Acid Morphogen Gradients
139(26)
T.F. Schilling
J. Sosnik
Q. Nie
Introduction
140(1)
1 Challenges for Morphogen Gradient Studies
140(2)
2 Feedback Allows Retinoic Acid to Act as a Graded Morphogen
142(1)
3 Cyp26s as Key Regulators of Retinoic Acid Gradient Formation
143(3)
4 Visualizing the Retinoic Acid Gradient
146(4)
5 Crabps and Retinoic Acid Signal Robustness
150(1)
6 Sharpening Boundaries of Gene Expression in Response to Retinoic Acid Gradients
151(5)
7 Noise---Both Good and Bad
156(1)
8 Other Boundaries and Other Morphogens
157(8)
Conclusions and Perspectives
158(1)
Acknowledgments
159(1)
References
159(6)
Chapter 8 Using Fluorescent Lipids in Live Zebrafish Larvae: from Imaging Whole Animal Physiology to Subcellular Lipid Trafficking
165(14)
J.L. Anderson
J.D. Carten
S.A. Farber
Introduction
166(1)
The Need for Whole Animal Studies of Lipid Metabolism
166(2)
1 Forward Genetic Screening With Fluorescent Lipids
168(3)
2 Visualizing Lipid Metabolism Using BODIPY Fatty Acid Analogs
171(8)
Summary
174(1)
Acknowledgments
175(1)
References
175(4)
Chapter 9 Analysis of Cilia Structure and Function in Zebrafish
179(50)
E. Leventea
K. Hazime
C. Zhao
J. Malicki
Introduction
180(1)
1 Cilia in Zebrafish Organs
181(9)
2 Analytical Tools for Cilia Morphology and Motility
190(18)
3 Phenotypes of Cilia Mutants in Zebrafish
208(7)
4 Future Directions
215(14)
Acknowledgments
216(1)
References
216(13)
Chapter 10 Functional Calcium Imaging in Zebrafish Lateral-line Hair Cells
229(24)
Q.X. Zhang
X.J. He
H.C. Wong
K.S. Kindt
Introduction
230(1)
1 Calcium Indicator Selection and Comparison
231(6)
2 Imaging Systems and Optimal Parameters
237(5)
3 Image Processing
242(11)
Summary
248(1)
Discussion
248(1)
Acknowledgments
249(1)
References
249(4)
Chapter 11 Physiological Recordings from the Zebrafish Lateral Line
253(28)
J. Olt
A.J. Ordoobadi
W. Marcotti
J.G. Trapani
Introduction
254(1)
1 Common Methods for Lateral Line Electrophysiology
255(3)
2 Stimulation of Neuromast Hair Cells
258(3)
3 Recording Microphonic Potentials
261(4)
4 In Vivo Hair Cell Physiology
265(5)
5 Afferent Neuron Action Currents
270(5)
6 Summary
275(6)
Discussion
275(1)
Acknowledgments
276(1)
References
276(5)
Volumes in Series 281(14)
Index 295
Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms. Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA Grousbeck Professor of Pediatrics, Boston Children's Hospital / HHMI, Boston, MA, USA