Contributors |
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xi | |
Preface |
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xv | |
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Chapter 1 Embryonic Cell Culture in Zebrafish |
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1 | (10) |
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2 | (1) |
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3 | (8) |
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10 | (1) |
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10 | (1) |
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Chapter 2 Cellular Dissection of Zebrafish Hematopoiesis |
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11 | (44) |
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12 | (1) |
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1 Zebrafish Hematopoiesis |
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12 | (12) |
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2 Hematopoietic Cell Transplantation |
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24 | (8) |
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32 | (3) |
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4 In Vitro Culture and Differentiation of Hematopoietic Progenitors |
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35 | (20) |
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46 | (1) |
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46 | (9) |
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Chapter 3 Second Harmonic Generation Microscopy in Zebrafish |
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55 | (14) |
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56 | (3) |
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59 | (3) |
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62 | (4) |
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66 | (3) |
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66 | (1) |
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67 | (1) |
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67 | (2) |
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Chapter 4 Imaging Blood Vessels and Lymphatic Vessels in the Zebrafish |
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69 | (36) |
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70 | (1) |
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1 Imaging Vascular Gene Expression |
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71 | (3) |
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2 Nonvital Blood Vessel and Lymphatic Vessel Imaging |
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74 | (9) |
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3 Vital Imaging of Blood and Lymphatic Vessels |
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83 | (22) |
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98 | (1) |
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99 | (6) |
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Chapter 5 An Eye on Light-sheet Microscopy |
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105 | (20) |
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106 | (1) |
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106 | (1) |
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1 Principle Behind Selective Plane Illumination Microscopy |
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107 | (7) |
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2 The Microscope for Your Sample or the Sample for Your Microscope? |
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114 | (3) |
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3 Data Acquisition and Handling |
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117 | (2) |
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4 Challenges and Perspectives |
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119 | (6) |
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120 | (1) |
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121 | (4) |
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Chapter 6 Single Neuron Morphology In Vivo with Confined Primed Conversion |
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125 | (14) |
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126 | (1) |
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1 Photoconvertible Fluorescent Proteins |
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127 | (1) |
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2 Confined Primed Conversion |
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128 | (1) |
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3 Unraveling Single Neuron Morphology With Confined Primed Conversion |
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129 | (10) |
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135 | (1) |
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136 | (3) |
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Chapter 7 Visualizing Retinoic Acid Morphogen Gradients |
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139 | (26) |
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140 | (1) |
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1 Challenges for Morphogen Gradient Studies |
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140 | (2) |
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2 Feedback Allows Retinoic Acid to Act as a Graded Morphogen |
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142 | (1) |
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3 Cyp26s as Key Regulators of Retinoic Acid Gradient Formation |
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143 | (3) |
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4 Visualizing the Retinoic Acid Gradient |
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146 | (4) |
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5 Crabps and Retinoic Acid Signal Robustness |
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150 | (1) |
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6 Sharpening Boundaries of Gene Expression in Response to Retinoic Acid Gradients |
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151 | (5) |
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7 Noise---Both Good and Bad |
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156 | (1) |
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8 Other Boundaries and Other Morphogens |
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157 | (8) |
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Conclusions and Perspectives |
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158 | (1) |
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159 | (1) |
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159 | (6) |
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Chapter 8 Using Fluorescent Lipids in Live Zebrafish Larvae: from Imaging Whole Animal Physiology to Subcellular Lipid Trafficking |
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165 | (14) |
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166 | (1) |
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The Need for Whole Animal Studies of Lipid Metabolism |
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166 | (2) |
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1 Forward Genetic Screening With Fluorescent Lipids |
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168 | (3) |
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2 Visualizing Lipid Metabolism Using BODIPY Fatty Acid Analogs |
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171 | (8) |
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174 | (1) |
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175 | (1) |
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175 | (4) |
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Chapter 9 Analysis of Cilia Structure and Function in Zebrafish |
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179 | (50) |
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180 | (1) |
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1 Cilia in Zebrafish Organs |
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181 | (9) |
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2 Analytical Tools for Cilia Morphology and Motility |
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190 | (18) |
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3 Phenotypes of Cilia Mutants in Zebrafish |
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208 | (7) |
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215 | (14) |
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216 | (1) |
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216 | (13) |
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Chapter 10 Functional Calcium Imaging in Zebrafish Lateral-line Hair Cells |
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229 | (24) |
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230 | (1) |
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1 Calcium Indicator Selection and Comparison |
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231 | (6) |
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2 Imaging Systems and Optimal Parameters |
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237 | (5) |
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242 | (11) |
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248 | (1) |
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248 | (1) |
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249 | (1) |
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249 | (4) |
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Chapter 11 Physiological Recordings from the Zebrafish Lateral Line |
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253 | (28) |
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254 | (1) |
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1 Common Methods for Lateral Line Electrophysiology |
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255 | (3) |
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2 Stimulation of Neuromast Hair Cells |
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258 | (3) |
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3 Recording Microphonic Potentials |
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261 | (4) |
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4 In Vivo Hair Cell Physiology |
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265 | (5) |
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5 Afferent Neuron Action Currents |
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270 | (5) |
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275 | (6) |
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275 | (1) |
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276 | (1) |
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276 | (5) |
Volumes in Series |
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281 | (14) |
Index |
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295 | |