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Zebrafish: Disease Models and Chemical Screens 4th edition, Volume 138 [Kietas viršelis]

Volume editor H. William Detrich III (Professor of Biochemistry and Marine Biology at Northeastern University), Volume editor Monte Westerfield (Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA), Volume editor Leonard Zon (Boston Children's Hospital / HHMI, Boston, MA, USA)

Formatas: Hardback, 746 pages, aukštis x plotis: 235x191 mm, weight: 1700 g
Serija: Methods in Cell Biology
Išleidimo metai: 27-Jan-2017
Leidėjas: Academic Press Inc
ISBN-10: 0128034734
ISBN-13: 9780128034736

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Cellular biology (cytology) - (Šiuo metu turimos knygos: 1 prekės)

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Formatas: Hardback, 746 pages, aukštis x plotis: 235x191 mm, weight: 1700 g
Serija: Methods in Cell Biology
Išleidimo metai: 27-Jan-2017
Leidėjas: Academic Press Inc
ISBN-10: 0128034734
ISBN-13: 9780128034736

Kitos knygos pagal šią temą:

Fishes (ichthyology) - (Šiuo metu turimos knygos: 1 prekės)
Cellular biology (cytology) - (Šiuo metu turimos knygos: 1 prekės)

Pastovi nuoroda: https://www.kriso.lt/db/9780128034736.html

The Zebrafish: Disease Models and Chemical Screens, Fourth Edition, the latest volume in the Methods in Cell Biology series, looks at methods for analyzing genetics, genomics, and transcriptomics of zebrafish. Chapters cover such topics as gene-trap mutagenesis, genetic screens for mutations, gene editing in zebrafish, homologous gene targeting, genome-wide RNA tomography, developmental epigenetics, and the zebrafish interactome.

Covers sections on model systems and functional studies, imaging-based approaches, and emerging studies
Includes chapters written by experts in the field
Contains cutting-edge material on the topics discussed

Recenzijos

Praise for the Series: "The series is invaluable for workers at all levels of cell biology." --Nature

Daugiau informacijos

This new volume in the Methods in Cell Biology series looks at methods for the genetics, genomics, and transcriptomics of zebrafish

Contributors

xvii

Preface

xxiii

PART I ADIPOSE TISSUE

Chapter 1 In Vivo Imaging and Quantification of Regional Adiposity in Zebrafish

(28)

J.E.N. Minchin

J.F. Rawls

Introduction

(1)

1 Rationale

(2)

2 Materials

(4)

3 Methods

(12)

4 Summary

(8)

Acknowledgments

(1)

References

(7)

PART II INNATE AND ADAPTIVE IMMUNE SYSTEMS

Chapter 2 Innate Immune Cells and Bacterial Infection in Zebrafish

(30)

J.W. Astin

P. Keerthisinghe

L. Du

L.E. Sanderson

K.E. Crosier

P.S. Crosier

C.J. Hall

Introduction

(3)

1 Quantifying the Innate Immune Cell Response to Bacterial Infection

(7)

2 Bioassays for Assessing Neutrophil Bactericidal Function

(4)

3 Bioassays for Assessing Macrophage Bactericidal Function

(4)

4 Drug Discovery Platform to Identify New Immunomodulatory Drugs

(11)

Acknowledgments

(1)

References

(6)

Chapter 3 Best Practices for Germ-Free Derivation and Gnotobiotic Zebrafish Husbandry

(40)

E. Melancon

S. Gomez De La Torre Canny

S. Sichel

M. Kelly

T.J. Wiles

J.F. Rawls

J.S. Eisen

K. Guillemin

Introduction

(4)

1 Experimental Procedures

(23)

2 Prospectus

(4)

3 Solutions

(8)

Acknowledgments

(1)

References

(6)

Chapter 4 Infectious Disease Models in Zebrafish

101

(36)

C. Sullivan

M.A. Matty

D. Jurczyszak

K.A. Gabor

P.J. Millard

D.M. Tobin

C.H. Kim

Introduction

103

(1)

1 Methods for Systemic Bacterial and Viral Infections

104

(19)

2 Methods for Localized Bacterial and Viral Infections

123

(14)

Conclusion

128

(1)

References

129

(8)

Chapter 5 Live Imaging the Earliest Host Innate Immune Response to Preneoplastic Cells Using a Zebrafish Inducible KalTA4-ERT2/UAS System

137

(14)

D.W. Laux

L. Kelly

I. Ribeiro Bravo

T. Ramezani

Y. Feng

Introduction

138

(3)

1 Generation of Preneoplastic Cell Clones Using the KalTA4-ERT2/UAS System in Zebrafish Larvae

141

(2)

2 Live Imaging Preneoplastic Cell: Neutrophil Interaction Using Confocal Microscopy

143

(2)

3 Image Analysis and 4D Reconstruction

145

(6)

Conclusion

147

(1)

Acknowledgments

147

(1)

References

147

(4)

Chapter 6 Studying the Adaptive Immune System in Zebrafish by Transplantation of Hematopoietic Precursor Cells

151

(14)

N. Iwanami

I. Hess

M. Schorpp

T. Boehm

Introduction

152

(3)

1 Methodology for the Transplantation of Hematopoietic Cells

155

(2)

2 Discussion

157

(2)

3 Future Directions

159

(6)

Acknowledgments

159

(1)

References

160

(5)

PART III BLOOD AND LYMPH

Chapter 7 Hematopoietic Stem Cell Development: Using the Zebrafish to Identify Extrinsic and Intrinsic Mechanisms Regulating Hematopoiesis

165

(28)

J.M. Frame

S.-E. Lim

T.E. North

Introduction to Hematopoietic Development

166

(5)

1 Use of Zebrafish to Investigate Hematopoietic Stem Cell Development

171

(8)

2 Zebrafish Tools and Protocols

179

(14)

References

184

(9)

Chapter 8 Studying Disorders of Vertebrate Iron and Heme Metabolism Using Zebrafish

193

(28)

Lisa N. van der Vorm

Barry H. Paw

1 Overview of Vertebrate Cellular Iron and Heme Metabolism

194

(2)

2 Advantageous Properties of Zebrafish to Study Genetics

196

(1)

3 Tools to Study Iron and Heme Metabolism Using Zebrafish

197

(24)

Conclusions and Future Directions

212

(1)

Acknowledgments

212

(1)

References

212

(9)

Chapter 9 The Lymphatic Vasculature Revisited---New Developments in the Zebrafish

221

(20)

Y. Padberg

S. Schulte-Merker

A. van Impel

1 Development of the Lymphatic System in the Zebrafish Trunk

222

(2)

2 Molecular Mechanisms Regulating Lymphatic Cell Fate Specification

224

(3)

3 Vegfc Signaling and Sprouting From the Posterior Cardinal Vein

227

(2)

4 Parachordal Lymphangioblast Migration at the Level of the Horizontal Myoseptum

229

(2)

5 Development of Lymphatic Structures in the Head and the Gut

231

(10)

Concluding Remarks

233

(1)

References

233

(8)

PART IV VISCERAL ORGANS

Chapter 10 Modeling Intestinal Disorders Using Zebrafish

241

(30)

X. Zhao

M. Pack

1 Intestinal Development, Morphology, and Physiology

242

(3)

2 Intestinal Microbiota and Host---Microbe Interactions

245

(2)

3 Intestinal Inflammatory Conditions

247

(7)

4 Enteric Nervous System and Motility Disorders

254

(4)

5 Intestinal Tumorigenesis and Cancer

258

(13)

Concluding Remarks

259

(1)

Acknowledgments

260

(1)

References

260

(11)

Chapter 11 Analysis of Pancreatic Disease in Zebrafish

271

(28)

S.C. Eames Nalle

K.F. Franse

M.D. Kinkel

Introduction

272

(1)

1 Method
1. Fasting Adult Zebrafish

273

(2)

2 Method
2. Weighing Live, Swimming Zebrafish Without Anesthetic

275

(1)

3 Method
3. Glucose Delivery to Adult Zebrafish Using Intraperitoneal Injection

276

(9)

4 Method
4. Dissection of the Pancreas, En Bloc, for Histology

285

(3)

5 Method
5. Sterile Dissection and Culture of the Principal Islet

288

(11)

Acknowledgments

294

(1)

References

294

(5)

PART V MUSCULOSKELETAL SYSTEM

Chapter 12 Using the Zebrafish to Understand Tendon Development and Repair

299

(22)

J.W. Chen

J.L. Galloway

Introduction

300

(1)

1 Tendon Structure

301

(1)

2 Tendon Formation and Differentiation

301

(3)

3 Tissue Interactions Within the Developing Musculoskeletal System

304

(2)

4 Methods to Study the Embryonic Tendon Program in Zebrafish

306

(15)

Conclusion

314

(1)

Acknowledgments

314

(1)

References

315

(6)

Chapter 13 Small Teleost Fish Provide New Insights Into Human Skeletal Diseases

321

(26)

P.E. Witten

M.P. Harris

A. Huysseune

C. Winkler

Introduction

322

(1)

1 A Fresh View on the Teleost Skeleton and Its Special Characters

323

(4)

2 Analyzing Skeletal Phenotypes of Small Fish

327

(5)

3 Mutant and Transgenic Fish Open New Directions in Skeletal Research

332

(2)

4 Genetic Phenocopies of Human Skeletal Diseases

334

(1)

5 Lifelong Tooth Replacement

335

(12)

Concluding Remarks

336

(1)

Acknowledgments

337

(1)

References

337

(10)

Chapter 14 Muscular Dystrophy Modeling in Zebrafish

347

(36)

M. Li

K.J. Hromowyk

S.L. Amacher

P.O. Currie

Introduction

348

(2)

1 Muscular Dystrophies and the Dystrophin-Associated Glycoprotein Complex

350

(4)

2 Skeletal Muscle Properties in Zebrafish

354

(7)

3 Models of Muscular Dystrophy in Zebrafish

361

(5)

4 Small Molecule Screens

366

(17)

Conclusions

368

(1)

References

369

(14)

PART VI CENTRAL AND SENSORY NERVOUS SYSTEMS

Chapter 15 Analysis of Myelinated Axon Formation in Zebrafish

383

(32)

M. D'Rozario

K.R. Monk

S.C. Petersen

Introduction

384

(2)

1 Visualization of Myelinating Glia in Zebrafish

386

(6)

2 Genetic Analysis of Myelin Development in Zebrafish

392

(6)

3 Pharmacological Manipulation of Myelinated Axons in Zebrafish

398

(3)

4 Plasticity, Maintenance, and Regeneration of Myelinated Axons in Zebrafish

401

(14)

Conclusions

404

(1)

Acknowledgments

404

(1)

References

404

(11)

Chapter 16 Zebrafish Models of Human Eye and Inner Ear Diseases

415

(56)

B. Blanco-Sanchez

A. Clement

J.B. Phillips

M. Westerfield

Introduction

416

(3)

1 Zebrafish Models of Eye Disease

419

(4)

2 Zebrafish Models of Ear Disease

423

(9)

3 Zebrafish Models of Syndromes Affecting Eye and/or Ear

432

(39)

Conclusion

445

(1)

References

446

(25)

PART VII CANCER

Chapter 17 A Zebrafish Xenograft Model for Studying Human Cancer Stem Cells in Distant Metastasis and Therapy Response

471

(26)

L. Chen

A. Groenewoud

C. Tulotta

E. Zoni

M. Kruithof-de Julio

G. van der Horst

G. van der Pluijm

B. Ewa Snaar-Jagalska

Introduction

472

(2)

1 Establishment of Human Cancer Stem Cells Xenograft Model

474

(6)

2 Analysis of Interaction Between Cancer Stem/Progenitor-Like Cells and Microenvironment

480

(6)

3 Genetic and Chemical Targeting of Tumor-Stroma Interactions in Cancer Stem/Progenitor-Like Cells---Xenograft

486

(11)

Conclusion

489

(1)

Acknowledgments

490

(1)

References

490

(7)

Chapter 18 Zebrafish as a Model for Von Hippel Lindau and Hypoxia-inducible Factor Signaling

497

(28)

H.R. Kim

D. Greenald

A. Vettori

E. Markham

K. Santhakumar

F. Argenton

F. van Eeden

Introduction

498

(1)

1 Hypoxic Signaling

499

(1)

2 Hypoxic Signaling: Overview of the Zebrafish Orthologues

500

(1)

3 The Zebrafish HIF Genes

500

(1)

4 HIF Protein Expression

501

(3)

5 The HIF Hydroxylases

504

(1)

6 The VHL Genes

504

(1)

7 HIF Binding Sites in the Genome

505

(1)

8 Hypoxic/HIF Target Gene Comparison

506

(1)

9 Hypoxic Signaling Reporters in Zebrafish

507

(1)

10 Links Between HIF and the Circadian Clock

507

(1)

11 Zebrafish VHL Mutants as Models of Hif Hyperactivation

508

(1)

12 Hematopoietic and Angiogenic Phenotypes in VHL Mutants

509

(1)

13 VHL/HIF Effects on Metabolism

510

(1)

14 Kidney Defects in VHL Mutants and Cancer

511

(1)

15 Links Between VHL HIF and P53

512

(13)

Appendix A Supplementary data

514

(1)

References

514

(11)

Chapter 19 Discovering Novel Oncogenic Pathways and New Therapies Using Zebrafish Models of Sarcoma

525

(38)

M.N. Hayes

D.M. Langenau

Introduction

526

(3)

1 Rhabdomyosarcoma

529

(8)

2 Malignant Peripheral Nerve Sheath Tumors

537

(6)

3 Ewing's Sarcoma

543

(3)

4 Chordoma

546

(3)

5 Hemangiosarcoma

549

(2)

6 Liposarcoma

551

(3)

7 Future Perspectives and Opportunities

554

(9)

References

554

(9)

Chapter 20 Zebrafish Models of Leukemia

563

(30)

S. He

C.-B. Jing

A.T. Look

1 T-Cell Acute Lymphoblastic Leukemia

564

(8)

2 B-Cell Acute Lymphoblastic Leukemia

572

(2)

3 Myeloid Malignancies

574

(19)

Conclusions

581

(1)

References

581

(12)

Chapter 21 Investigating Microglia-Brain Tumor Cell Interactions In Vivo in the Larval Zebrafish Brain

593

(36)

K.R. Astell

D. Sieger

Introduction

594

(4)

1 Methods

598

(22)

2 Summary

620

(9)

Acknowledgments

620

(1)

References

621

(8)

PART VIII TRANSPLANTATION

Chapter 22 Transplantation in Zebrafish

629

(22)

J.M. Gansner

M. Dang

M. Ammerman

L.I. Zon

Introduction

630

(1)

1 Rationale

631

(3)

2 Methods

634

(7)

3 Discussion

641

(10)

Acknowledgments

643

(1)

References

643

(8)

PART IX CHEMICAL SCREENING

Chapter 23 Chemical Screening in Zebrafish for Novel Biological and Therapeutic Discovery

651

(30)

D.S. Wiley

S.E. Redfield

L.I. Zon

Introduction

652

(4)

1 Rationale

656

(1)

2 Materials and Methods

656

(13)

3 Discussion/caveats

669

(12)

Summary

671

(1)

Acknowledgments

672

(1)

References

672

(9)

Index

681

(28)

Volumes in Series

709

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms. Grousbeck Professor of Pediatrics, Boston Children's Hospital / HHMI, Boston, MA, USA Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA

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