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Zebrafish: Methods and Protocols 2009 ed. [Minkštas viršelis]

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  • Formatas: Paperback / softback, 335 pages, aukštis x plotis: 260x193 mm, weight: 759 g, XI, 335 p., 1 Paperback / softback
  • Serija: Methods in Molecular Biology 546
  • Išleidimo metai: 27-Nov-2014
  • Leidėjas: Humana Press Inc.
  • ISBN-10: 162703899X
  • ISBN-13: 9781627038997
Kitos knygos pagal šią temą:
Zebrafish: Methods and Protocols 2009 ed.Didesnis paveikslėlis
  • Formatas: Paperback / softback, 335 pages, aukštis x plotis: 260x193 mm, weight: 759 g, XI, 335 p., 1 Paperback / softback
  • Serija: Methods in Molecular Biology 546
  • Išleidimo metai: 27-Nov-2014
  • Leidėjas: Humana Press Inc.
  • ISBN-10: 162703899X
  • ISBN-13: 9781627038997
Kitos knygos pagal šią temą:
Pastovi nuoroda: https://www.kriso.lt/db/9781627038997.html
Raktažodžiai:

In the last 20 years, research activity using the zebrafish Danio rerio has increased dramatically, due in part to the ease of breeding and raising them, their genetic tractability, embryonic accessibility, and their imaging potential. InZebrafish: Methods and Protocols, expert researchers provide a collection of experimental techniques for the genetic modification, manipulation, and in vivo live imaging of zebrafish. This volume collects sophisticated and practical techniques, including several not yet widely known, from laboratories around the world. Written in the highly successfulMethods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Zebrafish: Methods and Protocols seeks to aid and inspire scientists utilizing this versatile animal model in their research.



In the last 20 years, research activity using the zebrafish Danio rerio has increased dramatically. In this book, experts provide a collection of experimental techniques for the genetic modification, manipulation, and in vivo live imaging of zebrafish.

Preface v
Contributors ix
Part I Mutagenesis and Mutants
1 Highly Efficient ENU Mutagenesis in Zebrafish
Ewart de Bruijn, Edwin Cuppen, and Harma Feitsma
3(10)
2 Production of Pseudotyped Retrovirus and the Generation of Proviral Transgenic Zebrafish
Li-En Jao and Shawn M. Burgess
13(18)
3 Undertaking a Successful Gynogenetic Haploid Screen in Zebrafish
Judith E. Layton
31(14)
4 Cryopreservation and In Vitro Fertilization at the Zebrafish International Resource Center
Carrie Carmichael, Monte Westerfield, and Zoltan M. Varga
45(24)
Part II Transgenesis
5 Transient and Stable Transgenesis Using Tol2 Transposon Vectors
Hiroshi Kikuta and Koichi Kawakami
69(16)
6 Analysis of Genes and Genome by the Tol2-Mediated Gene and Enhancer Trap Methods
Akihiro Urasaki and Koichi Kawakami
85(18)
7 Bacterial Artificial Chromosome Transgenesis for Zebrafish
Zhongan Yang, Hong Jiang, and Shuo Lin
103(14)
8 Simple and Efficient Transgenesis with Meganuclease Constructs in Zebrafish
Daniele Soroldoni, Benjamin M. Hogan, and Andrew C. Oates
117(16)
Part III Tissue-Specific Manipulations
9 Nitroreductase-Mediated Cell Ablation in Transgenic Zebrafish Embryos
Harshan Pisharath and Michael J. Parsons
133(12)
10 Focal Electroporation in Zebrafish Embryos and Larvae
Marcel Tawk, Isaac H. Bianco, and Jonathan D. W. Clarke
145(8)
11 Tissue Micromanipulation in Zebrafish Embryos
Alexander Picker, Daniela Rollig, Olivier Pourquie, Andrew C. Oates, and Michael Brand
153(22)
Part IV ANALYZING GENE EXPRESSION
12 Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments. Part I: Array Printing and Hybridization
Siew Hong Lam, Sinnakarupan Mathavan, and Zhiyuan Gong
175(22)
13 Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments: Data Acquisition and Analysis
Siew Hong Lam, R. Krishna Murthy Karuturi, and Zhiyuan Gong
197(30)
14 Validating microRNA Target Transcripts Using Zebrafish Assays
Luke Pase and Graham J. Lieschke
227(16)
Part V IMAGING
15 Multiple Embryo Time-Lapse Imaging of Zebrafish Development
Leah Herrgen, Christian Schroter, Lola Bajard, and Andrew C. Oates
243(12)
16 Live Cell Imaging of Zebrafish Leukocytes
Chris Hall, Maria Vega Flores, Kathy Crosier, and Phil Crosier
255(18)
17 Imaging Zebrafish Embryos by Two-Photon Excitation Time-Lapse Microscopy
Lara Carvalho and Carl-Philipp Heisenberg
273(16)
18 Morphological Analysis of the Zebrafish Digestive System
Andrew J. Trotter, Adam C. Parslow, and Joan K. Heath
289(28)
19 In Toto Imaging of Embryogenesis with Confocal Time-Lapse Microscopy
Sean G. Megason
317(1)
Index 317