The zebrafish is fast becoming the vertebrate model of choice in biomedical research. This book is written by facility managers and researchers, with daily hands-on experience of husbandry and experimental implementation of this species.
After securing popularity in home aquariums, zebrafish have also become an essential animal for biomedical research. Ensuring they are adequately cared for in research settings is therefore of the utmost importance and this book is designed to help laboratories develop good welfare and husbandry practices, refine procedural protocols and engage with ethical obligations.
A wide range of topics are addressed, starting with husbandry aspects such as environmental enrichment, nutrition, housing conditions, diseases, health monitoring, biosecurity and larval rearing. More experimental topics are then discussed, such as genotyping, ageing, imaging, behavioral studies, experimental design and statistics. Ethical challenges are also considered through the environmental impact of aquatic facilities and the 3Rs - Replacement, Reduction and Refinement - with a focus on anaesthesia, analgesia, euthanasia and severity assessment.
Zebrafish are a shoaling species by nature but this book also takes into account their individual needs by providing:
- practical advice from experienced zebrafish caretakers;
- bridges between data acquisition and fish welfare; and
- the latest guidance for reporting, reproducibility and compliance.
This book is written for zebrafish facility personnel and for researchers who are aware of the need for robust care for their zebrafish model. It also provides an unparalleled insight for ethics committee members with the intention to provide the best care possible for this ever-important fish.
Daugiau informacijos
This book is suitable for researchers and students of aquaculture and related subjects
The zebrafish model--Claire Allenand and Jean-Philippe Mocho
Environmental enrichment for zebrafish--Mollie E. Millington, Christian Lawrence, Lynne U. Sneddon and Claire Allen
Dietary requirements for zebrafish--Joana Monteiro, Sandra Martins, Rita Almeida, Carolina Cabrera and Ana Catarina Certal
Non-infectious diseases of zebrafish--Katrina N. Murray and Michael L. Kent
Infectious diseases of zebrafish--Joanna Cambray-Young
Adult zebrafish as a vertebrate model of ageing--Pam S. Ellis, Raquel R. Martins and Catarina M. Henriques
Organization of the zebrafish facility--Jean-Philippe Mocho
The 3Rs in zebrafish research--Kamar E. Ameen-Ali and Claire Allen
Statistical considerations for the breeding of zebrafish and their use in experiments--Amy Spencer, Peter Laud, Claire Allen, James Bull, Eleanor Stillman and Sara Hilditch
Genotyping methods in zebrafish--Claire Allen
Imaging of zebrafish--Karishma Chhabria
Behaviour in zebrafish--Raquel R. Martins and Paul G. Schroeder
Anaesthesia, analgesia and euthanasia of zebrafish--Jean-Philippe Mocho
Severity assignment for EU and UK procedures on Zebrafish--Jean-Philippe Mocho
Jean-Philippe Mocho (Edited By) J-P graduated in France as a Doctor in Veterinary Medicine at the start of the century. He practiced as a large animal vet across the French countryside, Wales, Scotland, and Northern Ireland. In 2009, he joined the Royal Veterinary College to dedicate his career to laboratory animals as a Named Veterinary Surgeon, providing his services to London's major academia and research institutes. His approach based on his experience of population medicine led him to the new shores of aquatic facilities. He devoted himself to the development of better care for zebrafish, thanks to experimental data supporting the 3Rs and the publication of recommendations. The latter was helped by J-P's leadership in activities of the Federation of European Laboratory Animal Science Associations, such as organising international congresses, chairing scientific committees, and convening working groups on aquatics.
