Baculovirus Expression Protocols, Second Edition, provides the detailed steps required to perform the techniques involved with the use of baculoviruses and insect cell culture and discusses problems that may be encountered. This newly-revised and updated edition provides a cutting edge step-by-step guide for biochemists, molecular biologists, biochemical engineers and others using the BEVS and/or insect cells for producing recombinant proteins. Furthermore, Baculovirus Expression Protocols, Second Edition, provides assistance to scientists and engineers interested in developing and producing baculovirus insecticides. Authors list all available insect cell lines and provide methods for isolating new cell lines. The procedures involved in producing products, both at laboratory scale and large scale, are discussed, as well as the production in insect larvae. It is hoped that this volume will not only aid the user in successfully completing the tasks described herein, but will also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture. Timely and thorough, this volume will become an essential reference for anyone using baculoviruses and insect cell culture.
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2nd edition
Table of Contents PART I. INTRODUCTION
Chapter 1 Useful Tips, Widely Used Techniques and Quantifying Cell Metabolic Behavior David W. Murhammer PART II. BACULOVIRUS MOLECULAR BIOLOGY/DEVELOPMENT OF RECOMBINANT BACULOVIRUSES
Chapter 2 Introduction to Baculovirus Molecular Biology Barbara J. Kelly, Linda A. King, and Robert D. Possee
Chapter 3 Baculovirus Transfer Vectors Robert D. Possee and Linda A. King
Chapter 4 Recombinant Baculovirus Isolation Linda A. King, Richard Hitchman, and Robert D. Possee
Chapter 5 Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System James A. Fornwald, Quinn Lu, Dayuan Wang, and Robert S. Ames PART III. INSECT CELL CULTURE
Chapter 6 Available Lepidopteran Insect Cell Lines Dwight E. Lynn
Chapter 7 Lepidopteran Insect Cell Line Isolation From Insect Tissue Dwight E. Lynn
Chapter 8 Development of Serum-Free Media for Lepidopteran Insect Cell Lines Sprios N. Agathos
Chapter 9 Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculoviruses Dwight E. Lynn PART IV. PROTEIN PRODUCTION WITH RECOMBINANT BACULOVIRUSES
Chapter 10 Small Scale Protein Production With the Baculovirus Expression Vector System Steve Harwood
Chapter 11 Recombinant Protein Production in Large Scale Agitated Bioreactors Using the Baculovirus Expression Vector System Cynthia B. Elias, Barbara Jardin, and Amine Kamen
Chapter 12 Production of Baculovirus-Expressed Recombinant Proteins in Wave Bioreactors Sue H. Kadwell and Philip I. Hardwicke
Chapter 13 Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae Yi Liu, Nathan DeCarolis, and Nikolai van Beek
Chapter 14 Virus-Like Particle and Viral Vector Production Using the BEVS/Insect Cell System: Adeno-Associated Virus Based Products Marc G. Aucoin, Danielle Jacob, Parminder S. Chahal, Jamal Meghrous, Alice Bernier, and Amine A. Kamen PART V. RECOMBINANT PROTEIN PRODUCTION WITH TRANSFORMED INSECT CELLS
Chapter 15 Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression Robert L. Harrison and Donald L Jarvis
Chapter 16 Transformation of Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression Lucy Cherbas and Peter Cherbas
Chapter 17 Transforming Lepidopteran Insect Cells for Improved Protein Processing Robert L. Harrison and Donald L Jarvis PART VI. BACULOVIRUS DEVELOPMENT AND PRODUCTION FOR USE AS INSECTICIDES
Chapter 18 Introduction to the Use of Baculoviruses as Biological Insecticides Bryony C. Bonning and Tyasning Nusawardani
Chapter 19 Baculovirus Insecticide Production in Insect Larvae Nikolai van Beek and David C. Davis
Chapter 20 Evaluation of the Insecticidal Efficacy of Wild Type and Recombinant Baculoviruses Huarong Li and Bryony C. Bonning PART VII. MISCELLANEOUS TECHNIQUES AND APPLICATIONS OF THE BACULOVIRUS/INSECT CELL SYSTEM
Chapter 21 Monitoring and Visualization of Baculovirus Infection Using Green Fluorescent Protein Strategy Hyung Joon Cha and William E. Bentley
Chapter 22 Alternative Molecular and Reactor Strategies Probing Infection and Production Yu-Chen Hu and William E. Bentley
Chapter 23 Methods For Gene Silencing with RNAi John C. March and William E. Bentley
Chapter 24 Use of the Baculovirus/Insect Cell System to Study Apoptosis Nor Chejanovsky
Chapter 25 Generation of Envelope-Modified Baculoviruses for Gene Delivery Into Mammalian Cells Christian Hofmann
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