Baculovirus and Insect Cell Expression Protocols Second Edition 2007 [Kietas viršelis]

Murhammer (chemical and biochemical engineering, University of Iowa) details steps for techniques used in baculovirus and insect cell culture in this guide for biochemists, molecular biologists, and biochemical engineers using baculovirus expression vector systems (BEVs) and/or insect cells for producing recombinant proteins. An introductory chapter overviews techniques discussed in the book and offers step-by-step instructions for quantifying cell growth, baculovirus infection, and cell metabolism. Contributors list all available insect cell lines, provide methods for isolating new cell lines, and cover the production of insect larvae, at both laboratory and large scales. Material is in sections on baculovirus molecular biology and development of recombinant baculoviruses; insect cell culture; protein production with recombinant baculoviruses; recombinant protein production with transformed insect cells; baculovirus development and production for use as insecticides; and miscellaneous techniques and applications. Annotation ©2007 Book News, Inc., Portland, OR (booknews.com)

Baculovirus Expression Protocols, Second Edition, provides the detailed steps required to perform the techniques involved with the use of baculoviruses and insect cell culture and discusses problems that may be encountered. This newly-revised and updated edition provides a cutting edge step-by-step guide for biochemists, molecular biologists, biochemical engineers and others using the BEVS and/or insect cells for producing recombinant proteins. Furthermore, Baculovirus Expression Protocols, Second Edition, provides assistance to scientists and engineers interested in developing and producing baculovirus insecticides. Authors list all available insect cell lines and provide methods for isolating new cell lines. The procedures involved in producing products, both at laboratory scale and large scale, are discussed, as well as the production in insect larvae. It is hoped that this volume will not only aid the user in successfully completing the tasks described herein, but will also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture. Timely and thorough, this volume will become an essential reference for anyone using baculoviruses and insect cell culture.

Baculovirus Expression Protocols, Second Edition, provides the detailed steps required to perform the techniques involved with the use of baculoviruses and insect cell culture and discusses problems that may be encountered. This newly-revised and updated edition provides a cutting edge step-by-step guide for biochemists, molecular biologists, biochemical engineers and others using the BEVS and/or insect cells for producing recombinant proteins. Furthermore, Baculovirus Expression Protocols, Second Edition, provides assistance to scientists and engineers interested in developing and producing baculovirus insecticides. Authors list all available insect cell lines and provide methods for isolating new cell lines. The procedures involved in producing products, both at laboratory scale and large scale, are discussed, as well as the production in insect larvae. It is hoped that this volume will not only aid the user in successfully completing the tasks described herein, but will also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture. Timely and thorough, this volume will become an essential reference for anyone using baculoviruses and insect cell culture.