Atnaujinkite slapukų nuostatas

Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols 1st ed. 2016 [Kietas viršelis]

Edited by , Edited by
  • Formatas: Hardback, 234 pages, aukštis x plotis: 254x178 mm, weight: 6148 g, 42 Illustrations, color; 30 Illustrations, black and white; XI, 234 p. 72 illus., 42 illus. in color., 1 Hardback
  • Serija: Methods in Molecular Biology 1466
  • Išleidimo metai: 30-Jul-2016
  • Leidėjas: Humana Press Inc.
  • ISBN-10: 1493940120
  • ISBN-13: 9781493940127
Kitos knygos pagal šią temą:
  • Formatas: Hardback, 234 pages, aukštis x plotis: 254x178 mm, weight: 6148 g, 42 Illustrations, color; 30 Illustrations, black and white; XI, 234 p. 72 illus., 42 illus. in color., 1 Hardback
  • Serija: Methods in Molecular Biology 1466
  • Išleidimo metai: 30-Jul-2016
  • Leidėjas: Humana Press Inc.
  • ISBN-10: 1493940120
  • ISBN-13: 9781493940127
Kitos knygos pagal šią temą:
This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study.
Preface v
Contributors ix
1 Chemical and Instrumental Approaches for Capillary Electrophoresis (CE)--Fluorescence Analysis of Proteins
1(10)
Isabelle Le Potter
Audrey Boutonnet
Vincent Ecochard
Francois Couderc
2 Discrimination of Glycoproteins from Unglycosylated Proteins in Capillary Electrophoresis: Two-Color LIF Detection Coupled with Post-column Derivatization
11(14)
Takashi Kaneta
3 Capillary Zone Electrophoresis-Mass Spectrometry of Intact Proteins
25(18)
Elena Dominguez-Vega
Rob Haselberg
Govert W. Somsen
4 Screening of Small Intact Proteins by Capillary Electrophoresis Electrospray Ionization-Mass Spectrometry (CE-ESI-MS)
43(14)
Sabine Neuberger
Angelina Rafai
Christian Neusuß
5 Online Capillary IsoElectric Focusing-ElectroSpray Ionization Mass Spectrometry (CIEF-ESI MS) in Glycerol--Water Media for the Separation and Characterization of Hydrophilic and Hydrophobic Proteins
57(10)
Meriem Mokaddem
Fanny d'Orlye
Anne Varenne
6 On-Line Solid-Phase Extraction Capillary Electrophoresis Mass Spectrometry for Preconcentration and Clean-Up of Peptides and Proteins
67(18)
Fernando Benavente
Silvia Medina-Casanellas
Estela Gimenez
Victoria Sanz-Nebot
7 Affinity Monolith-Integrated Microchips for Protein Purification and Concentration
85(8)
Changlu Gao
Xinhua Sun
Huaixin Wang
Wei Qiao
Bo Hu
8 Analysis of Somatropin by Double-Injection Capillary-Zone Electrophoresis in Polybrene/Chondroitin Sulfate A Double-Coated Capillaries
93(14)
Ahmad Amini
9 Poly(N, N-Dimethylacrylamide)-Based Coatings to Modulate Electroosmotic Flow and Capillary Surface Properties for Protein Analysis
107(14)
Laura Sola
Marina Cretich
Marcella Chiari
10 Measurement of Inflammatory Chemokines in Micro-dissected Tissue Biopsy Samples by Chip-Based Immunoaffinity Capillary Electrophoresis
121(16)
Terry M. Phillips
Edward Wellner
Shane McMohan
Heather Kalish
11 Separation of Recombinant Therapeutic Proteins Using Capillary Gel Electrophoresis and Capillary Isoelectric Focusing
137(14)
Caitlyn A. G. De Jong
Jessica Risley
Alexis K. Lee
Shuai Sherry Zhao
David D. Y. Chen
12 Characterization of Chemical and Physical Modifications of Human Serum Albumin by Capillary Zone Electrophoresis
151(14)
Anne-Lise Marie
Nguyet Thuy Tran
Myriam Taverna
13 Capillary Electrophoresis Method for the Assessment of Erythropoiesis-Stimulating Agents in Final Formulations
165(14)
Michel Girard
Anita Kane
Sylvie Boucher
14 Quality Control of Therapeutic Monoclonal Antibodies at the Hospital After Their Compounding and Before Their Administration to Patients
179(6)
Emmanuel Jaccoulet
Claire Smadja
Myriam Taverna
15 Capillary Electrophoresis-Ultraviolet-Mass Spectrometry (CE-UV-MS) for the Simultaneous Determination and Quantification of Insulin Formulations
185(12)
Julie Schappler
Serge Rudaz
16 Applications of an Automated and Quantitative CE-Based Size and Charge Western Blot for Therapeutic Proteins and Vaccines
197(22)
Richard R. Rustandi
Melissa Hamm
Catherine Lancaster
John W. Loughney
17 Affinity Capillary Electrophoresis Applied to Investigation of Valinomycin Complexes with Ammonium and Alkali Metal Ions
219(14)
Sille Stepanova
Vaclav Kasicka
Index 233