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Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols Softcover reprint of the original 1st ed. 2016 [Minkštas viršelis]

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  • Formatas: Paperback / softback, 234 pages, aukštis x plotis: 254x178 mm, weight: 4728 g, 42 Illustrations, color; 30 Illustrations, black and white; XI, 234 p. 72 illus., 42 illus. in color., 1 Paperback / softback
  • Serija: Methods in Molecular Biology 1466
  • Išleidimo metai: 30-May-2018
  • Leidėjas: Humana Press Inc.
  • ISBN-10: 1493981536
  • ISBN-13: 9781493981533
Kitos knygos pagal šią temą:
  • Formatas: Paperback / softback, 234 pages, aukštis x plotis: 254x178 mm, weight: 4728 g, 42 Illustrations, color; 30 Illustrations, black and white; XI, 234 p. 72 illus., 42 illus. in color., 1 Paperback / softback
  • Serija: Methods in Molecular Biology 1466
  • Išleidimo metai: 30-May-2018
  • Leidėjas: Humana Press Inc.
  • ISBN-10: 1493981536
  • ISBN-13: 9781493981533
Kitos knygos pagal šią temą:
This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study.
Chemical and Instrumental Approaches for Capillary Electrophoresis
(CE)-Fluorescence Analysis of Proteins.- Discrimination of Glycoproteins from
Unglycosylated Proteins in Capillary Electrophoresis: Two-Color LIF Detection
Coupled with Post-Column Derivatization.- Capillary Zone Electrophoresis-Mass
Spectrometry of Intact Proteins.- Screening of Small Intact Proteins by
Capillary Electrophoresis Electrospray Ionization-Mass Spectrometry
(CE-ESI-MS).- Online Capillary IsoElectric Focusing-ElectroSpray Ionization
Mass Spectrometry (CIEF-ESI MS) in Glycerol-Water Media for the Separation
and Characterization of Hydrophilic and Hydrophobic Proteins.- On-Line
Solid-Phase Extraction Capillary Electrophoresis Mass Spectrometry for
Preconcentration and Clean-Up of Peptides and Proteins.- Affinity
Monolith-Integrated Microchips for Protein Purification and
Concentration.- Analysis of Somatropin by Double Injection Capillary Zone
Electrophoresis in Polybrene/Chondroitin Sulfate A Double Coated
Capillaries.- Poly(N,N-Dimethylacrylamide)-Based Coatings to Modulate
Electroosmotic Flow and Capillary Surface Properties for Protein
Analysis.- Measurement of Inflammatory Chemokines in Micro-Dissected Tissue
Biopsy Samples by Chip-Based Immunoaffinity Capillary
Electrophoresis.- Separation of Recombinant Therapeutic Proteins Using
Capillary Gel Electrophoresis and Capillary Isoelectric
Focusing.- Characterization of Chemical and Physical Modifications of Human
Serum Albumin by Capillary Zone Electrophoresis.- Capillary Electrophoresis
Method for the Assessment of Erythropoiesis Stimulating Agents in Final
Formulations.- Quality Control of Therapeutic Monoclonal Antibodies at the
Hospital After Their Compounding and Before Their Administration to
Patients.- Capillary Electrophoresis Ultraviolet Mass Spectrometry (CE-UV-MS)
for the Simultaneous Determination and Quantitation of Insulin
Formulations.- Applications of an Automated and Quantitative CE-Based Size
and Charge Western Blot for Therapeutic Proteins and Vaccines.- Affinity
Capillary Electrophoresis Applied to Investigation of Valinomycin Complexes
with Ammonium and Alkali Metal Ions.