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El. knyga: Plant Biochemistry

(Professor, Institute for Biological Sciences, University of Rostock, Germany), (Professor, University Goettingen, Germany)
  • Formatas: PDF+DRM
  • Išleidimo metai: 07-Oct-2004
  • Leidėjas: Academic Press Inc
  • Kalba: eng
  • ISBN-13: 9780080473772
Kitos knygos pagal šią temą:
  • Formatas: PDF+DRM
  • Išleidimo metai: 07-Oct-2004
  • Leidėjas: Academic Press Inc
  • Kalba: eng
  • ISBN-13: 9780080473772
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This fully revised translation of the world-renowned German edition covers the complete and modern knowledge of plant biochemistry. The book presents the topic in a concise and simplified manner so that students can digest the message and gain a basic knowledge of the entire field of plant biochemistry, from photosynthesis (the synthesis of natural plant products) to all kinds of genetic engineering with its many commercial applications. Topics include cell structure and function, lipid and polysaccharide metabolism, nitrogen fixation, phloem transport, synthesis and function of isoprenoids, phenylpropanoids and other secondary metabolites, plant growth regulation and development. Heldt provides a comprehensive description of photosynthesis, primary and secondary metabolism, the function of phytohormones and molecular engineering. The text covers research findings until the end of 2003 and identifies areas of future research. There are extensive references to the most recent scientific literature.

* Illustrated with two-color diagrams and well designed metabolic schemes
* Plant metabolism presented in the context of the structure and function of the plant
* Many examples of commercial applications for the protection and quality improvement of harvest crops, plant breeding, and the production of raw materials for industrial use
* Coverage of the latest research findings

Recenzijos

"Plant Biochemistry will be an invaluable resource for students, teachers and researchers looking for a clear, up to date presentation which covers the essentials without being overwhelmed by details. There are simple, easy to grasp illustrations throughout....I would highly recommend this book as a text in plant biochemistry, physiology and biotechnology courses. The book highlights examples on how an understanding of plant biochemistry is fundamental towards the applications of biotechnology, which are bringing about a revolution in agriculture." --Gerald Edwards, Washington State University

"This is a welcome addition to the sparsely populated topic of recent textbooks in plant biochemistry. It complements rather than unnecessarily duplicates the many fine general biochemistry volumes available...The contents are well organized and laid out with logical and informative section titles. The text is nicely illustrated and regularly gives a clear and easily understandable overview of complex subjects, as well as provides some additional detail...This book will be an excellent introduction and overview of plant biochemistry to anyone interested in the subject. It also provides a nice reference for students of pharmacy, nutrition, environmental sciences, and ecology, as well as general biology students. As such, it will be a good textbook for classes associated with these subjects." --David Hildebrand, Agronomy, University of Kentucky in QUARTERLY REVIEW OF BIOLOGY

"The book Plant Biochemistry and Molecular Biology by Professor Hans Heldt is an outstanding text. The material is elegantly presented, with attention to molecular structures, intracellular compartmentation, and regulatory mechanisms controlling metabolism. Difficult topics are presented with incredible clarity and with a style that reflects many decades of cutting-edge research and effective teaching by the author. I highly recommend this book for both students and researchers in the field of plant biochemistry." --Steve Huber, University of Illinois

"The third edition of Plant Biochemistry by Hans-Walter Heldt updates my favorite text on this topic. My students prefered the first edition of this book to more encyclopedic treatments by a wide margin. The book covers aspects of biochemistry that are essential to a broad understanding of plant function. The book is focused on clear explanations of the essential concepts rather than an encyclopedic approach. Simple diagrams make even complex topics clear. The typeface and layout of the new edition is improved, with important words in bold, making important topics stand out for easy comprehension. This book is invaluable as a text for any plant biochemistry course." --Thomas Sharkey, University of Wisconsin

"This 3rd edition of H.W. Heldt's Plant Biochemistry textbook is built around proven artwork from previous editions, with some intelligent additions. It adds substantial new text and more references than in the past that can guide the reader to in-depth studies, often with references from 2004, clearly this textbook is not outdated. This edition places much emphasis on concepts that are presented in lucid fashion, providing an insight into complex processes in a straightforward way. This is particularly obvious in those areas of plant biochemistry that have been the author's own field of research for several decades. This textbook is an excellent vehicle to make green biochemistry understandable to undergraduate students." --Hans Bohnert, University of Illinois

Daugiau informacijos

* Illustrated with two-color diagrams and well designed metabolic schemes * Plant metabolism presented in the context of the structure and function of the plant * Many examples of commercial applications for the protection and quality improvement of harvest crops, plant breeding, and the production of raw materials for industrial use * Coverage of the latest research findings
A leaf cell consists of several metabolic compartments
1(44)
The cell wall gives the plant cell mechanical stability
4(5)
The cell wall consists mainly of carbohydrates and proteins
4(3)
Plasmodesmata connect neighboring cells
7(2)
Vacuoles have multiple functions
9(1)
Plastids have evolved from cyanobacteria
10(5)
Mitochondria also result from endosymbionts
15(1)
Peroxisomes are the site of reactions in which toxic intermediates are formed
16(2)
The endoplasmic reticulum and Golgi apparatus form a network for the distribution of biosynthesis products
18(4)
Functionally intact cell organelles can be isolated from plant cells
22(2)
Various transport processes facilitate the exchange of metabolites between different compartments
24(2)
Translocators catalyze the specific transport of substrates and products of metabolism
26(6)
Translocators have a common basic structure
29(2)
Aquaporins make cell membranes permeable for water
31(1)
Ion channels have a very high transport capacity
32(5)
Porins consist of β-sheet structures
37(8)
Further reading
40(5)
The use of energy from sunlight by photosynthesis is the basis of life on earth
45(22)
How did photosynthesis start?
45(2)
Pigments capture energy from sunlight
47(5)
The energy content of light depends on its wavelength
47(2)
Chlorophyll is the main photosynthetic pigment
49(3)
Light absorption excites the chlorophyll molecule
52(4)
The return of the chlorophyll molecule from the first singlet state to the ground state can proceed in different ways
55(1)
An antenna is required to capture light
56(11)
How is the excitation energy of the photons, which have been captured in the antennae, transferred to the reaction centers?
58(1)
The function of an antenna can be illustrated using the antenna of photosystem II as an example
59(3)
Phycobilisomes enable cyanobacteria and red algae to carry out photosynthesis even in dim light
62(4)
Further reading
66(1)
Photosynthesis is an electron transport process
67(48)
The photosynthetic machinery is constructed from modules
67(4)
A reductant and an oxidant are formed during photosynthesis
71(1)
The basic structure of a photosynthetic reaction center has been resolved by X-ray structure analysis
72(5)
X-ray structure analysis of the photosynthetic reaction center
74(1)
The reaction center of Rhodopseudomonas viridis has a symmetric structure
75(2)
How does a reaction center function?
77(4)
Two photosynthetic reaction centers are arranged in tandem in photosynthesis of algae and plants
81(3)
Water is split by photosystem II
84(8)
Photosystem II complex is very similar to the reaction center in purple bacteria
88(2)
Mechanized agriculture usually necessitates the use of herbicides
90(2)
The cytochrome-b6/f complex mediates electron transport between photosystem II and photosystem I
92(7)
Iron atoms in cytochromes and in iron-sulfur centers have a central function as redox carriers
92(3)
The electron transport by the cytochrome-b6/f complex is coupled to a proton transport
95(3)
The number of protons pumped through the cyt-b6/f complex can be doubled by a Q-cycle
98(1)
Photosystem I reduces NADP
99(5)
In cyclic electron transport by PS I light energy is used for the synthesis of ATP only
103(1)
In the absence of other acceptors electrons can be transferred from photosystem I to oxygen
104(4)
Regulatory processes control the distribution of the captured photons between the two photosystems
108(7)
Excess light energy is eliminated as heat
110(2)
Further reading
112(3)
ATP is generated by photosynthesis
115(20)
A proton gradient serves as an energy-rich intermediate state during ATP synthesis
116(3)
The electron chemical proton gradient can be dissipated by uncouplers to heat
119(2)
The chemiosmotic hypothesis was proved experimentally
121(1)
H+-ATP synthases from bacteria, chloroplasts, and mitochondria have a common basic structure
121(6)
X-ray structure analysis of the F1 part of ATP synthase yields an insight into the machinery of ATP synthesis
125(2)
The synthesis of ATP is effected by a conformation change of the protein
127(8)
In photosynthetic electron transport the stoichiometry between the formation of NADPH and ATP is still a matter of debate
130(1)
H+-ATP synthase of chloroplasts is regulated by light
130(1)
V-ATPase is related to the F-ATP synthase
131(1)
Further reading
132(3)
Mitochondria are the power station of the cell
135(30)
Biological oxidation is preceded by a degradation of substrates to form bound hydrogen and CO2
135(1)
Mitochondria are the sites of cell respiration
136(2)
Mitochondria form a separated metabolic compartment
137(1)
Degradation of substrates for biological oxidation takes place in the matrix compartment
138(7)
Pyruvate is oxidized by a multienzyme complex
138(3)
Acetate is completely oxidized in the citrate cycle
141(3)
A loss of intermediates of the citrate cycle is replenished by anaplerotic reactions
144(1)
How much energy can be gained by the oxidation of NADH?
145(2)
The mitochondrial respiratory chain shares common features with the photosynthetic electron transport chain
147(6)
The complexes of the mitochondrial respiratory chain
149(4)
Electron transport of the respiratory chain is coupled to the synthesis of ATP via proton transport
153(4)
Mitochondrial proton transport results in the formation of a membrane potential
155(1)
Mitochondrial ATP synthesis serves the energy demand of the cytosol
156(1)
Plant mitochondria have special metabolic functions
157(3)
Mitochondria can oxidize surplus NADH without forming ATP
158(1)
NADH and NADPH from the cytosol can be oxidized by the respiratory chain of plant mitochondria
159(1)
Compartmentation of mitochondrial metabolism requires specific membrane translocators
160(5)
Further reading
162(3)
The Calvin cycle catalyzes photosynthetic CO2 assimilation
165(30)
CO2 assimilation proceeds via the dark reaction of photosynthesis
166(2)
Ribulose bisphosphate carboxylase catalyzes the fixation of CO2
168(6)
The oxygenation of ribulose bisphosphate: a costly side-reaction
170(2)
Ribulose bisphosphate carboxylase/oxygenase: special features
172(1)
Activation of ribulose bisphosphate carboxylase/oxygenase
172(2)
The reduction of 3-phosphoglycerate yields triose phosphate
174(2)
Ribulose bisphosphate is regenerated from triose phosphate
176(7)
Besides the reductive pentose phosphate pathway there is also an oxidative pentose phosphate pathway
183(4)
Reductive and oxidative pentose phosphate pathways are regulated
187(8)
Reduced thioredoxins transmit the signal for ``illumination'' to enzyme proteins
187(2)
The thioredoxin modulated activation of chloroplast enzymes releases an inbuilt blockage
189(1)
An abundance of further regulatory processes ensures that the various steps of the reductive pentose phosphate pathway are matched
190(2)
Further reading
192(3)
In the photorespiratory pathway phosphoglycolate formed by the oxygenase activity of RubisCo is recycled
195(18)
Ribulose 1,5-bisphosphate is recovered by recycling 2-phosphoglycolate
195(6)
The NH4+ released in the photorespiratory pathway is refixed in the chloroplasts
201(2)
For the reduction of hydroxypyruvate, peroxisomes have to be provided with external reducing equivalents
203(4)
Reducing equivalents are taken up into the peroxisomes via a malate-oxaloacetate shuttle
203(2)
Mitochondria export reducing equivalents via a malate-oxaloacetate shuttle
205(1)
A ``malate valve'' controls the export of reducing equivalents from the chloroplasts
205(2)
The peroxisomal matrix is a special compartment for the disposal of toxic products
207(1)
How high are the costs of the ribulose bisphosphate oxygenase reaction for the plant?
208(1)
There is no net CO2 fixation at the compensation point
209(1)
The photorespiratory pathway, although energy-consuming, may also have a useful function for the plant
210(3)
Further reading
211(2)
Photosynthesis implies the consumption of water
213(30)
The uptake of CO2 into the leaf is accompanied by an escape of water vapor
213(2)
Stomata regulate the gas exchange of a leaf
215(4)
Malate plays an important role in guard cell metabolism
215(2)
Complex regulation governs stomatal opening
217(2)
The diffusive flux of CO2 into a plant cell
219(3)
C4 plants perform CO2 assimilation with less water consumption than C3 plants
222(13)
The CO2 pump in C4 plants
223(2)
C4 metabolism of the NADP-malic enzyme type plants
225(4)
C4 metabolism of the NAD-malic enzyme type
229(2)
C4 metabolism of the phosphoenolpyruvate carboxykinase type
231(2)
Kranz-anatomy with its mesophyll and bundle sheath cells is not an obligatory requirement for C4 metabolism
233(1)
Enzymes of C4 metabolism are regulated by light
233(1)
Products of C4 metabolism can be identified by mass spectrometry
234(1)
C4 plants include important crop plants but also many of the worst weeds
234(1)
Crassulacean acid metabolism makes it possible for plants to survive even during a very severe water shortage
235(8)
CO2 fixed during the night is stored in the form of malic acid
236(2)
Photosynthesis proceeds with closed stomata
238(2)
C4 as well as CAM metabolism has been developed several times during evolution
240(1)
Further reading
240(3)
Polysaccharides are storage and transport forms of carbohydrates produced by photosynthesis
243(32)
Starch and sucrose are the main products of CO2 assimilation in many plants
244(1)
Large quantities of carbohydrate can be stored as starch in the cell
244(11)
Starch is synthesized via ADP-glucose
248(2)
Degradation of starch proceeds in two different ways
250(3)
Surplus photosynthesis products can be stored temporarily in chloroplasts by starch synthesis
253(2)
Sucrose synthesis takes place in the cytosol
255(2)
The utilization of the photosynthesis product triose phosphate is strictly regulated
257(6)
Fructose 1,6-bisphosphatase functions as an entrance valve for the pathway of sucrose synthesis
257(4)
Sucrose phosphate synthase is regulated not only by metabolites but also by covalent modification
261(1)
Partitioning of assimilates between sucrose and starch is due to the interplay of several regulatory mechanisms
262(1)
In some plants assimilates from the leaves are exported as sugar alcohols or oligosaccharides of the raffinose family
263(2)
Fructans are deposited as storage substances in the vacuole
265(4)
Cellulose is synthesized by enzymes located in the plasma membrane
269(6)
Synthesis of callose is often induced by wounding
271(1)
Cell wall polysaccharides are also synthesized in the Golgi apparatus
271(1)
Further reading
271(4)
Nitrate assimilation is essential for the synthesis of organic matter
275(34)
The reduction of nitrate to NH3 proceeds in two partial reactions
276(6)
Nitrate is reduced to nitrite in the cytosol
278(1)
The reduction of nitrite to ammonia proceeds in the plastids
279(1)
The fixation of NH4+ proceeds in the same way as in photorespiration
280(2)
Nitrate assimilation also takes place in the roots
282(2)
The oxidative pentose phosphate pathway provides reducing equivalents for nitrite reduction in leucoplasts
282(2)
Nitrate assimilation is strictly controlled
284(4)
The synthesis of the nitrate reductase protein is regulated at the level of gene expression
285(1)
Nitrate reductase is also regulated by reversible covalent modification
285(1)
14-3-3 Proteins are important metabolic regulators
286(1)
The regulation of nitrate reductase and of sucrose phosphate synthase have great similarities
287(1)
The end-product of nitrate assimilation is a whole spectrum of amino acids
288(14)
CO2 assimilation provides the carbon skeletons to synthesize the end-products of nitrate assimilation
288(2)
The synthesis of glutamate requires the participation of mitochondrial metabolism
290(1)
Biosynthesis of proline and arginine
291(2)
Aspartate is the precursor of five amino acids
293(2)
Acetolactate synthase participates in the synthesis of hydrophobic amino acids
295(4)
Aromatic amino acids are synthesized via the shikimate pathway
299(1)
Glyphosate acts as an herbicide
299(2)
A large proportion of the total plant matter can be formed by the shikimate pathway
301(1)
Glutamate is precursor for synthesis of chlorophylls and cytochromes
302(7)
Protophorphyrin is also a precursor for heme synthesis
304(2)
Further reading
306(3)
Nitrogen fixation enables the nitrogen in the air to be used for plant growth
309(16)
Legumes form a symbiosis with nodule-forming bacteria
310(8)
The formation of nodules is due to a regulated interplay of the expression of specific bacteria and plant genes
313(1)
Metabolic products are exchanged between bacteroids and host cells
313(2)
Nitrogenase reductase delivers electrons for the nitrogenase reaction
315(1)
N2 as well as H+ are reduced by dinitrogenase
316(2)
N2 fixation can proceed only at very low oxygen concentrations
318(2)
The energy costs for utilizing N2 as a nitrogen source are much higher than for the utilization of NO3
320(1)
Plants improve their nutrition by symbiosis with fungi
320(2)
The arbuscular mycorrhiza is widespread
321(1)
Ectomycorrhiza supplies trees with nutrients
322(1)
Root nodule symbioses may have evolved from a preexisting pathway for the formation of arbuscular mycorrhiza
322(3)
Further reading
323(2)
Sulfate assimilation enables the synthesis of sulfur-containing substances
325(14)
Sulfate assimilation proceeds primarily by photosynthesis
325(5)
Sulfate assimilation has some parallels to nitrogen assimilation
326(1)
Sulfate is activated prior to reduction
327(1)
Sulfite reductase is similar to nitrite reductase
328(1)
H2S is fixed in the form of cysteine
329(1)
Glutathione serves the cell as an antioxidant and is an agent for the detoxification of pollutants
330(3)
Xenobiotics are detoxified by conjugation
331(1)
Phytochelatins protect the plant against heavy metals
332(1)
Methionine is synthesized from cysteine
333(2)
S-Adenosylmethionine is a universal methylation reagent
334(1)
Excessive concentrations of sulfur dioxide in air are toxic for plants
335(4)
Further reading
336(3)
Phloem transport distributes photoassimilates to the various sites of consumption and storage
339(14)
There are two modes of phloem loading
341(2)
Phloem transport proceeds by mass flow
343(1)
Sink tissues are supplied by phloem unloading
344(9)
Starch is deposited in plastids
345(1)
The glycolysis pathway plays a central role in the utilization of carbohydrates
345(5)
Further reading
350(3)
Products of nitrate assimilation are deposited in plants as storage proteins
353(10)
Globulins are the most abundant storage proteins
354(1)
Prolamins are formed as storage proteins in grasses
355(1)
2S-Proteins are present in seeds of dicot plants
356(1)
Special proteins protect seeds from being eaten by animals
356(1)
Synthesis of the storage proteins occurs at the rough endoplasmic reticulum
357(3)
Proteinases mobilize the amino acids deposited in storage proteins
360(3)
Further reading
360(3)
Glycerolipids are membrane constituents and function as carbon stores
363(40)
Polar glycerolipids are important membrane constituents
364(5)
The fluidity of the membrane is governed by the proportion of unsaturated fatty acids and the content of sterols
365(2)
Membrane lipids contain a variety of hydrophilic head groups
367(1)
Sphingolipids are important constituents of the plasma membrane
368(1)
Triacylglycerols are storage substances
369(3)
The de novo synthesis of fatty acids takes place in the plastids
372(10)
Acetyl CoA is the precursor for the synthesis of fatty acids
372(3)
Acetyl CoA carboxylase is the first enzyme of fatty acid synthesis
375(2)
Further steps of fatty acid synthesis are also catalyzed by a multienzyme complex
377(2)
The first double bond in a newly formed fatty acid is formed by a soluble desaturase
379(3)
Acyl-ACP formed as product of fatty acid synthesis in the plastids serves two purposes
382(1)
Glycerol 3-phosphate is a precursor for the synthesis of glycerolipids
382(6)
The ER membrane is the site of fatty acid elongation and desaturation
385(1)
Some of the plastid membrane lipids are formed via the eukaryotic pathway
386(2)
Triacylglycerols are formed in the membranes of the endoplasmic reticulum
388(4)
Plant fat is used not only for nutrition but also as a raw material in industry
389(1)
Plant fats are customized by genetic engineering
390(2)
During seed germination, storage lipids are mobilized for the production of carbohydrates in the glyoxysomes
392(4)
The glyoxylate cycle enables plants to synthesize hexoses from acetyl CoA
393(2)
Reactions with toxic intermediates take place in peroxisomes
395(1)
Lipoxygenase is involved in the synthesis of oxylipins, which are acting as defense and signal substances
396(7)
Further reading
401(2)
Secondary metabolites fulfill specific ecological functions in plants
403(10)
Secondary metabolites often protect plants from pathogenic microorganisms and herbivores
403(3)
Microbes can be pathogens
404(1)
Plants form phytoalexins in response to microbial infection
404(1)
Plant defense substances can also be a risk for humans
405(1)
Alkaloids comprise a variety of heterocyclic secondary metabolites
406(2)
Some plants emit prussic acid when wounded by animals
408(1)
Some wounded plants emit volatile mustard oils
409(1)
Plants protect themselves by tricking herbivores with false amino acids
410(3)
Further reading
411(2)
A large diversity of isoprenoids has multiple functions in plant metabolism
413(22)
Higher plants have two different synthesis pathways for isoprenoids
415(3)
Acetyl CoA is the precursor for the synthesis of isoprenoids in the cytosol
415(2)
Pyruvate and D-glycerinaldehyde-3-phosphate are the precursors for the synthesis of isopentyl pyrophosphate in plastids
417(1)
Prenyl transferases catalyze the association of isoprene units
418(2)
Some plants emit isoprenes into the air
420(1)
Many aromatic substances are derived from geranyl pyrophosphate
421(2)
Farnesyl pyrophosphate is the precursor for the formation of sesquiterpenes
423(3)
Steroids are synthesized from farnesyl pyrophosphate
424(2)
Geranylgeranyl pyrophosphate is the precursor for defense substances, phytohormones, and carotenoids
426(2)
Oleoresins protect trees from parasites
426(1)
Carotene synthesis delivers pigments to plants and provides an important vitamin for humans
427(1)
A prenyl chain renders substances lipid-soluble
428(3)
Proteins can be anchored in a membrane by prenylation
429(1)
Dolichols mediate the glucosylation of proteins
430(1)
The regulation of isoprenoid synthesis
431(1)
Isoprenoids are very stable and persistent substances
431(4)
Further reading
432(3)
Phenylpropanoids comprise a multitude of plant secondary metabolites and cell wall components
435(20)
Phenylalanine ammonia lyase catalyzes the initial reaction of phenylpropanoid metabolism
437(1)
Monooxygenases are involved in the synthesis of phenols
438(2)
Phenylpropanoid compounds polymerize to macromolecules
440(6)
Lignans act as defense substances
442(1)
Lignin is formed by radical polymerization of phenylpropanoid derivatives
443(1)
Suberins form gas- and water-impermeable layers between cells
444(2)
Cutin is a gas- and water-impermeable constituent of the cuticle
446(1)
For the synthesis of flavonoids and stilbenes a second aromatic ring is formed from acetate residues
446(2)
The stilbenes include very potent natural fungicides
446(2)
Flavonoids have multiple functions in plants
448(2)
Anthocyanins are flower pigments and protect plants against excessive light
450(1)
Tannins bind tightly to proteins and therefore have defense functions
451(4)
Further reading
453(2)
Multiple signals regulate the growth and development of plant organs and enable their adaptation to environmental conditions
455(36)
Signal chains known from animal metabolism also function in plants
456(8)
G-proteins act as molecular switches
456(1)
Small G-proteins have diverse regulatory functions
457(1)
Ca++ acts as a messenger in signal transduction
458(1)
The phosphoinositol pathway controls the opening of Ca++ channels
459(2)
Calmodulin mediates the messenger function of Ca++ ions
461(1)
Phosphorylated proteins form elements of signal transduction
462(2)
Phytohormones comprise a variety of very different compounds
464(1)
Auxin stimulates shoot elongation growth
465(3)
Gibberellins regulate stem elongation
468(3)
Cytokinins stimulate cell division
471(2)
Abscisic acid controls the water balance of the plant
473(1)
Ethylene makes fruit ripen
474(2)
Plants also contain steroid and peptide hormones
476(3)
Brassinosteroids control plant development
476(2)
Various phytohormones are polypeptides
478(1)
Systemin induces defense against herbivore attack
478(1)
Phytosulfokines regulate cell proliferation
479(1)
Defense reactions are triggered by the interplay of several signals
479(2)
Light sensors regulate growth and development of plants
481(10)
Further reading
485(6)
A plant cell has three different genomes
491(40)
In the nucleus the genetic information is divided among several chromosomes
492(3)
The DNA sequences of plant nuclear genomes have been analyzed in a dicot and a monocot plant
495(1)
The DNA of the nuclear genome is transcribed by three specialized RNA polymerases
495(10)
The transcription of structural genes is regulated
496(1)
Promoter and regulatory sequences regulate the transcription of genes
497(1)
Transcription factors regulate the transcription of a gene
498(1)
Micro-RNAs inhibit gene expression by inactivating messeger RNAs
498(1)
The transcription of structural genes requires a complex transcription apparatus
499(2)
The formation of the messenger RNA requires processing
501(2)
rRNA and tRNA are synthesized by RNA polymerase I and III
503(2)
DNA polymorphism yields genetic markers for plant breeding
505(6)
Individuals of the same species can be differentiated by restriction fragment length polymorphism
506(2)
The RAPD technique is a simple method for investigating DNA polymorphism
508(3)
The polymorphism of micro-satellite DNA is used as a genetic marker
511(1)
Transposable DNA elements roam through the genome
511(2)
Most plant cells contain viruses
513(3)
Retrotransposons are degenerated retroviruses
515(1)
Plastids possess a circular genome
516(4)
The transcription apparatus of the plastids resembles that of bacteria
520(1)
The mitochondrial genome of plants varies largely in its size
520(11)
Mitochondrial DNA contains incorrect information that is corrected after transcription
524(1)
Male sterility of plants caused by the mitochondria is an important tool in hybrid breeding
525(4)
Further reading
529(2)
Protein biosynthesis occurs at different sites of a cell
531(26)
Protein synthesis is catalyzed by ribosomes
532(6)
A peptide chain is synthesized
534(3)
Specific inhibitors of the translation can be used to decide whether a protein is encoded in the nucleus or the genome of plastids or mitochondria
537(1)
The translation is regulated
537(1)
Proteins attain their three-dimensional structure by controlled folding
538(6)
The folding of a protein is a multistep process
539(1)
Proteins are protected during the folding process
540(1)
Heat shock proteins protect against heat damage
541(1)
Chaperones bind to unfolded proteins
541(3)
Nuclearly encoded proteins are distributed throughout various cell compartments
544(7)
Most of the proteins imported into the mitochondria have to cross two membranes
545(2)
The import of proteins into chloroplasts requires several translocation complexes
547(3)
Proteins are imported into peroxisomes in the folded state
550(1)
Proteins are degraded in a strictly controlled manner by proteasomes
551(6)
Further reading
554(3)
Gene technology makes it possible to alter plants to meet requirements of agriculture, nutrition, and industry
557(38)
A gene is isolated
558(10)
A gene library is required for the isolation of a gene
558(2)
A gene library can be kept in phages
560(2)
A gene library can also be kept in plasmids
562(1)
A gene library is screened for a certain gene
563(1)
A clone is identified by antibodies against the gene product
563(2)
A clone can also be identified by DNA probes
565(1)
Genes encoding unknown proteins can be isolated by complementation
566(2)
Genes can be tracked down with the help of transposons or T-DNA
568(1)
Agrobacteria have the ability to transform plant cells
568(4)
The Ti plasmid contains the genetic information for tumor formation
570(2)
Ti plasmids are used as transformation vectors
572(9)
A new plant is regenerated following transformation of a leaf cell
575(2)
Plants can be transformed by a modified shotgun
577(1)
Protoplasts can be transformed by the uptake of DNA
578(1)
The use of plastid transformation for genetic engineering of plants is of advantage for the environment
579(2)
Selection of appropriate promoters enables the defined expression of an inserted gene
581(1)
Gene products are directed into certain subcellular compartments by targeting sequences
582(1)
Genes can be turned off by transformation
582(3)
Plant genetic engineering can be used for many different purposes
585(10)
Plants are selectively protected against some insects by the BT protein
585(2)
Plants can be protected against viruses by gene technology
587(1)
The generation of fungus-resistant plants is still at an early stage
588(1)
Nonselective herbicides can be used as selective herbicide following the generation of herbicide-resistant plants
588(1)
Plant genetic engineering is used for the improvement of the yield and quality of crop products
589(1)
Genetic engineering is used to produce raw material for industry and pharmaceuticals
589(1)
Genetic engineering provides a chance for increasing the protection of crop plants against environmental stress
590(1)
The introduction of transgenic cultivars requires a risk analysis
591(1)
Further reading
591(4)
Index 595


Hans-Walter Heldt was a professor at the University of Göttingen in the Department of Biochemistry of the plant. He is co-authored over 250 scientific publications and is the co-author of the textbook, Plant Biochemistry. In 1993, he was awarded the Max Planck Research Award together with Marshall Davidson Hatch . Since 1990, he has been a full member of the Göttingen Academy of Sciences. Birgit Piechullas current research focus is to understand the biosynthesis and regulation of volatile organic compounds of bacteria and plants (flowers) and to elucidate the underlying molecular and cellular mechanisms and reactions, including signal transduction, in the mVOC receiver. She published 112 peer-reviewed publications, 25 book articles and non-peer-reviewed articles (Researchgate h-index 37). Her book, Plant Biochemistry, co-written with H.W. Heldt has been published in German, English, Russian, Japanese, Indian, Chinese and Turkish. From 1998 - 2013 she was co-editor of Plant Biology, board member of the Deutsche Botanische Gesellschaft (DBG) (2009-2016), board member of the Society of Biochemistry and Molecular Biology (GBM) (2008-2016), councilor of the Int. Society of Chemical Ecology (ISCE) (2014- 2017), and she held several academic positions within the university, including Vice Rector for Science at the University of Rostock (2,5 years, 2013-2015).