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El. knyga: Xenopus Protocols: Post-Genomic Approaches

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  • Formatas: PDF+DRM
  • Serija: Methods in Molecular Biology 917
  • Išleidimo metai: 06-Sep-2012
  • Leidėjas: Humana Press Inc.
  • Kalba: eng
  • ISBN-13: 9781617799921
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  • Formatas: PDF+DRM
  • Serija: Methods in Molecular Biology 917
  • Išleidimo metai: 06-Sep-2012
  • Leidėjas: Humana Press Inc.
  • Kalba: eng
  • ISBN-13: 9781617799921
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During the 20th century Xenopus has flourished as an accessible experimental model system for studying vertebrate biology. The second edition of Xenopus Protocols:Post-Genomic Approaches expands upon the previous edition with novel approaches inspired by Xenopus tropicalis and the sequencing of its genome. Xenopus tropicalis genomics and tractable genetics combined with the unique experimental advantages of Xenopus laevis and their distinctive phylogenetic position make them unique model systems for vertebrate embryonic development in the 21st century. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Xenopus Protocols: Post-Genomic Approaches, Second Edition provides a toolkit that will enable every laboratory to maximize the power of this extraordinary experimental system in the post-genomic age.

The 2nd edition of this Methods in Molecular Biology™ volume provides novel approaches inspired by Xenopus tropicalis and the sequencing of its genome. Offers a toolkit enabling laboratories to maximize the power of this extraordinary experimental system.
Preface v
Contributors xi
Part I Xenopus tropicalis
1 Xenopus tropicalis as a Model Organism for Genetics and Genomics: Past, Present, and Future
3(14)
Robert M. Grainger
2 Husbandry of Xenopus tropicalis
17(16)
Alan Jafkins
Anita Abu-Daya
Anna Noble
Lyle B. Zimmerman
Matthew Guille
3 Generating Diploid Embryos from Xenopus tropicalis
33(10)
Florencia del Viso
Mustafa Khokha
4 Navigating the Xenopus tropicalis Genome
43(26)
Ira L. Blitz
Part II Genetics in Xenopus
5 Genetic Analysis of Xenopus tropicalis
69(42)
Timothy J. Geach
Derek L. Stemple
Lyle B. Zimmerman
6 Forward Genetic Screens in Xenopus Using Transposon-Mediated Insertional Mutagenesis
111(18)
Donald A. Yergeau
Clair M. Kelley
Haiqing Zhu
Emin Kuliyev
Paul E. Mead
7 Targeted Gene Disruption with Engineered Zinc-Finger Nucleases (ZFNs)
129(14)
John J. Young
Richard M. Harland
8 Reverse Genetic Studies Using Antisense Morpholino Oligonucleotides
143(12)
Yanan Zhao
Shoko Ishibashi
Enrique Amaya
9 Chemical Genetics and Drug Discovery in Xenopus
155(12)
Matthew L. Tomlinson
Adam E. Hendry
Grant N. Wheeler
10 Maternal mRNA Knock-down Studies: Antisense Experiments Using the Host-Transfer Technique in Xenopus laevis and Xenopus tropicalis
167(18)
David J. Olson
Alissa M. Hulstrand
Douglas W. Houston
Part III Xenopus Trangenesis
11 Generating Transgenic Frog Embryos by Restriction Enzyme Mediated Integration (REMI)
185(20)
Shoko Ishibashi
Kristen L. Kroll
Enrique Amaya
12 A Simple Method of Transgenesis using I-Scel Meganuclease in Xenopus
205(14)
Shoko Ishibashi
Nick R. Love
Enrique Amaya
13 Using C31 Integrase to Mediate Insertion of DNA in Xenopus Embryos
219(12)
You E. Li
Bryan G. Allen
Daniel L. Weeks
14 Xenopus Transgenies: Methods Using Transposons
231(14)
Clair M. Kelley
Donald A. Yergeau
Haiqing Zhu
Emin Kuliyev
Paul E. Mead
15 Comparative Genomics-Based Identification and Analysis of Cis-Regulatory Elements
245(20)
Hajime Ogino
Haruki Ochi
Chihiro Uchiyama
Sarah Louie
Robert M. Grainger
16 Tet-On Binary Systems for Tissue-Specific and Inducible Transgene Expression
265(14)
Daniel R. Buchholz
Part IV Gene Expression: From Gene to Transcript to Protein
17 Chromatin Immunoprecipitation Analysis of Xenopus Embryos
279(14)
Robert C. Akkers
Ulrike G. Jacobi
Gert Jan C. Veenstra
18 Transcriptomics Using Next Generation Sequencing Technologies
293(26)
Dasfne Lee-Liu
Leonardo I. Almonacid
Fernando Faunes
Francisco Melo
Juan Larrain
19 Databases of Gene Expression in Xenopus Development
319(28)
Michael J. Gilchrist
Nicolas Pollet
20 Investigating Alternative RNA Splicing in Xenopus
347(22)
Agnes Mereau
Serge Hardy
21 Immunoisolation of Protein Complexes from Xenopus
369(22)
Frank L. Conlon
Yana Miteva
Erin Kaltenbrun
Lauren Waldron
Todd M. Greco
Heana M. Cristea
22 Complementary Protcomic Analysis of Protein Complexes
391(20)
Todd M. Greco
Uana Miteva
Frank L. Conlon
Ileana M. Cristea
Part V Imaging Xenopus Development
23 Antibody Development and Use in Chromogenic and Fluorescent Immunostaining
411(20)
Eamon Dubaissi
Niki Panagiotaki
Nancy Papalopulu
Peter D. Vize
24 Multiple Fluorescent In Situ mRNA Hybridization (FISH) on Whole Mounts and Sections
431(14)
Robert Lea
Boyan Bonev
Eamon Dubaissi
Peter D. Vize
Nancy Papalopulu
25 Methods to Analyze microRNA Expression and Function During Xenopus Development
445(16)
Boyan Bonev
Nancy Papalopulu
26 A Bromodeoxyuridine (BrdU) Based Protocol for Characterizing Proliferating Progenitors in Xenopus Embryos
461(16)
Helene Auger
Raphael Thuret
Warif El Yakoubi
Nancy Papalopulu
27 Microscopy Tools for Quantifying Developmental Dynamics in Xenopus Embryos
477(20)
Sagar D. Joshi
Hye Young Kim
Lance A. Davidson
Part VI Novel Approaches in Xenopus
28 Mathematical Modeling of Gene Regulatory Networks in Xenopus Development
497(18)
Yasushi Saka
29 Stem-Cell-Like Embryonic Explants to Study Cardiac Development
515(10)
Boni A. Afouda
30 Studying Regeneration in Xenopus
525(16)
Caroline W. Beck
31 On-Line Resources for Xenopus
541(22)
Jeff Bowes
Index 563