Preface |
|
v | |
Contributors |
|
xi | |
|
Part I Xenopus tropicalis |
|
|
|
1 Xenopus tropicalis as a Model Organism for Genetics and Genomics: Past, Present, and Future |
|
|
3 | (14) |
|
|
2 Husbandry of Xenopus tropicalis |
|
|
17 | (16) |
|
|
|
|
|
|
3 Generating Diploid Embryos from Xenopus tropicalis |
|
|
33 | (10) |
|
|
|
4 Navigating the Xenopus tropicalis Genome |
|
|
43 | (26) |
|
|
Part II Genetics in Xenopus |
|
|
|
5 Genetic Analysis of Xenopus tropicalis |
|
|
69 | (42) |
|
|
|
|
6 Forward Genetic Screens in Xenopus Using Transposon-Mediated Insertional Mutagenesis |
|
|
111 | (18) |
|
|
|
|
|
|
7 Targeted Gene Disruption with Engineered Zinc-Finger Nucleases (ZFNs) |
|
|
129 | (14) |
|
|
|
8 Reverse Genetic Studies Using Antisense Morpholino Oligonucleotides |
|
|
143 | (12) |
|
|
|
|
9 Chemical Genetics and Drug Discovery in Xenopus |
|
|
155 | (12) |
|
|
|
|
10 Maternal mRNA Knock-down Studies: Antisense Experiments Using the Host-Transfer Technique in Xenopus laevis and Xenopus tropicalis |
|
|
167 | (18) |
|
|
|
|
Part III Xenopus Trangenesis |
|
|
|
11 Generating Transgenic Frog Embryos by Restriction Enzyme Mediated Integration (REMI) |
|
|
185 | (20) |
|
|
|
|
12 A Simple Method of Transgenesis using I-Scel Meganuclease in Xenopus |
|
|
205 | (14) |
|
|
|
|
13 Using C31 Integrase to Mediate Insertion of DNA in Xenopus Embryos |
|
|
219 | (12) |
|
|
|
|
14 Xenopus Transgenies: Methods Using Transposons |
|
|
231 | (14) |
|
|
|
|
|
|
15 Comparative Genomics-Based Identification and Analysis of Cis-Regulatory Elements |
|
|
245 | (20) |
|
|
|
|
|
|
16 Tet-On Binary Systems for Tissue-Specific and Inducible Transgene Expression |
|
|
265 | (14) |
|
|
Part IV Gene Expression: From Gene to Transcript to Protein |
|
|
|
17 Chromatin Immunoprecipitation Analysis of Xenopus Embryos |
|
|
279 | (14) |
|
|
|
|
18 Transcriptomics Using Next Generation Sequencing Technologies |
|
|
293 | (26) |
|
|
|
|
|
|
19 Databases of Gene Expression in Xenopus Development |
|
|
319 | (28) |
|
|
|
20 Investigating Alternative RNA Splicing in Xenopus |
|
|
347 | (22) |
|
|
|
21 Immunoisolation of Protein Complexes from Xenopus |
|
|
369 | (22) |
|
|
|
|
|
|
|
22 Complementary Protcomic Analysis of Protein Complexes |
|
|
391 | (20) |
|
|
|
|
|
Part V Imaging Xenopus Development |
|
|
|
23 Antibody Development and Use in Chromogenic and Fluorescent Immunostaining |
|
|
411 | (20) |
|
|
|
|
|
24 Multiple Fluorescent In Situ mRNA Hybridization (FISH) on Whole Mounts and Sections |
|
|
431 | (14) |
|
|
|
|
|
|
25 Methods to Analyze microRNA Expression and Function During Xenopus Development |
|
|
445 | (16) |
|
|
|
26 A Bromodeoxyuridine (BrdU) Based Protocol for Characterizing Proliferating Progenitors in Xenopus Embryos |
|
|
461 | (16) |
|
|
|
|
|
27 Microscopy Tools for Quantifying Developmental Dynamics in Xenopus Embryos |
|
|
477 | (20) |
|
|
|
|
Part VI Novel Approaches in Xenopus |
|
|
|
28 Mathematical Modeling of Gene Regulatory Networks in Xenopus Development |
|
|
497 | (18) |
|
|
29 Stem-Cell-Like Embryonic Explants to Study Cardiac Development |
|
|
515 | (10) |
|
|
30 Studying Regeneration in Xenopus |
|
|
525 | (16) |
|
|
31 On-Line Resources for Xenopus |
|
|
541 | (22) |
|
Index |
|
563 | |