| Preface |
|
v | |
| Contributors |
|
xi | |
|
1 How It All Began: A Personal History of Gel Electrophoresis |
|
|
1 | (22) |
|
|
|
2 Introduction to Protein Electrophoresis |
|
|
23 | (6) |
|
|
|
3 Protein Quantification Methods to Determine Protein Concentration Prior to Electrophoresis |
|
|
29 | (8) |
|
|
|
4 Protein Solubilization: Attend to the Choice of Lysis Buffer |
|
|
37 | (12) |
|
|
|
|
|
|
|
5 Native Polyacrylamide Gels |
|
|
49 | (6) |
|
|
|
|
|
|
|
|
|
6 Cationic Electrophoresis |
|
|
55 | (10) |
|
|
|
7 SARCOSYL-PAGE: A New Electrophoretic Method for the Separation and Immunological Detection of PEGylated Proteins |
|
|
65 | (16) |
|
|
|
|
|
81 | (12) |
|
|
|
|
|
|
|
9 Analysis of Protein Glycation Using Phenylboronate Acrylamide Gel Electrophoresis |
|
|
93 | (18) |
|
|
|
|
|
|
|
|
|
10 Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins |
|
|
111 | (8) |
|
|
|
|
|
11 Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis |
|
|
119 | (16) |
|
|
|
|
|
12 A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples |
|
|
135 | (12) |
|
|
|
|
|
|
|
|
|
|
|
13 Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies |
|
|
147 | (26) |
|
|
|
14 Microchip Electrophoresis, with Respect to "Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheimer's Disease" |
|
|
173 | (12) |
|
|
|
|
|
|
|
|
|
|
|
15 A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols |
|
|
185 | (12) |
|
|
|
|
|
|
|
|
|
16 Peptide Fractionation by SDS-Free Polyacrylamide Gel Electrophoresis for Proteomic Analysis via DF-PAGE |
|
|
197 | (8) |
|
|
|
|
|
|
|
17 Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass |
|
|
205 | (10) |
|
|
|
|
|
|
|
|
|
|
|
|
|
18 SDS-PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting |
|
|
215 | (20) |
|
|
|
|
|
19 Two-Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing |
|
|
235 | (12) |
|
|
|
20 Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids |
|
|
247 | (12) |
|
|
|
21 Native Flatbed Isoelectric Focusing for Determining Antibody Clonotype Distribution |
|
|
259 | (8) |
|
|
|
|
|
22 Two-Dimensional Gel Electrophoresis: Glass Tube-Based IEF Followed by SDS-PAGE |
|
|
267 | (8) |
|
|
|
|
|
|
|
|
|
23 One-Dimensional and Two-Dimensional Immobilized Metal Affinity Electrophoresis |
|
|
275 | (12) |
|
|
|
|
|
|
|
|
|
|
|
24 Two-Dimensional Difference Gel Electrophoresis |
|
|
287 | (18) |
|
|
|
25 Protein Identification on Archived 2-D Gels |
|
|
305 | (4) |
|
|
|
|
|
|
|
26 Diagonal Electrophoresis for the Detection of Protein Disulfides |
|
|
309 | (8) |
|
|
|
27 Two-Dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes |
|
|
317 | (22) |
|
|
|
|
|
28 Immunoelectrophoresis: A Method with Many Faces |
|
|
339 | (22) |
|
|
|
29 Cell Surface Protein Biotinylation for SDS-PAGE Analysis |
|
|
361 | (12) |
|
|
|
30 A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization |
|
|
373 | (12) |
|
|
|
|
|
|
|
|
|
31 Gel-Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane Proteome |
|
|
385 | (8) |
|
|
|
|
|
32 Interactions of Hemoglobin in Live Red Blood Cells Measured by the Electrophoresis Release Test |
|
|
393 | (10) |
|
|
|
|
|
|
|
33 Extraction of Proteins from Gels: A Brief Review |
|
|
403 | (4) |
|
|
|
|
|
34 A New Algorithm for the Simulation of SDS 2D-PAGE Datasets |
|
|
407 | (20) |
|
|
|
|
|
35 Isolation of Proteins from Polyacrylamide Gels |
|
|
427 | (6) |
|
|
|
|
|
|
|
|
|
|
|
433 | (4) |
|
|
|
|
|
|
|
|
|
37 Long-Term, Buffer-Less, Wet Gel Storage in Non-sealed Polyethylene Bags |
|
|
437 | (8) |
|
|
|
|
|
38 A Brief Review of Other Notable Electrophoretic Methods |
|
|
445 | (6) |
|
|
|
39 Protein Stains and Applications |
|
|
451 | (14) |
|
|
|
Natarajan Balasubramaniyan |
|
|
|
|
40 Coomassie-Brilliant Blue Staining of Polyacrylamide Gels |
|
|
465 | (6) |
|
|
|
|
|
|
|
|
|
|
|
41 Accelerated Coomassie Blue Staining and Destaining of SDS-PAGE Gels with Application of Heat |
|
|
471 | (10) |
|
|
|
|
|
42 Silver Staining Techniques of Polyacrylamide Gels |
|
|
481 | (6) |
|
|
|
|
|
|
|
|
|
|
|
43 Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis |
|
|
487 | (10) |
|
|
|
44 Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry |
|
|
497 | (14) |
|
|
|
|
|
|
|
|
|
|
|
45 TEMED-Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots After PAGE |
|
|
511 | (10) |
|
|
|
|
|
46 Microwave-Assisted Protein Staining, Destaining, and In-Gel/In-Solution Digestion of Proteins |
|
|
521 | (12) |
|
|
|
|
|
47 Electrophoretic Separation and Detection of Metalloproteins by X-Ray Fluorescence Mapping |
|
|
533 | (10) |
|
|
|
|
|
|
|
48 Fluorescent Staining of Gels |
|
|
543 | (8) |
|
|
|
49 A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta During Western Blot Analysis |
|
|
551 | (10) |
|
|
|
|
|
|
|
50 Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain |
|
|
561 | (6) |
|
|
|
51 Spicy SDS-PAGE Gels: Curcumin/Turmeric as an Environment-Friendly Protein Stain |
|
|
567 | (12) |
|
|
|
|
|
|
|
52 In-Gel Staining of Proteins in Native Polyacrylamide Gel Electrophoresis Using Meso-Tetrakis(4-Sulfonatophenyl) Porphyrin |
|
|
579 | (6) |
|
|
|
|
|
|
|
53 Rapid Detection of Proteins in Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black |
|
|
585 | (6) |
|
|
|
|
|
|
|
54 Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gel |
|
|
591 | (16) |
|
Mircea Alexandru Mateescu |
|
|
|
|
|
|
|
|
|
|
55 Coomassie Brilliant Blue Removal/Disposal from Gel Destain and Used Gel Stain in an Environment-Friendly Manner |
|
|
607 | (10) |
|
|
|
|
|
56 A Brief Review of Other Notable Protein Detection Methods on Acrylamide Gels |
|
|
617 | (4) |
|
|
|
|
|
57 Ultrasensitive Protein Detection and Imaging: Comparison of Lumitein™, ProtcoSilver™, SYPRO Ruby, and Coomassic® Brilliant Blue Gel Stains |
|
|
621 | (12) |
|
|
|
|
|
|
|
58 Common Artifacts and Mistakes Made in Electrophorcsis |
|
|
633 | (8) |
|
|
|
|
| Index |
|
641 | |
