| Preface |
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| Contributors |
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1 How It All Began: A Personal History of Gel Electrophoresis |
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1 | (22) |
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2 Introduction to Protein Electrophoresis |
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23 | (6) |
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3 Protein Quantification Methods to Determine Protein Concentration Prior to Electrophoresis |
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29 | (8) |
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4 Protein Solubilization: Attend to the Choice of Lysis Buffer |
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37 | (12) |
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5 Native Polyacrylamide Gels |
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49 | (6) |
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6 Cationic Electrophoresis |
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55 | (10) |
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7 SARCOSYL-PAGE: A New Electrophoretic Method for the Separation and Immunological Detection of PEGylated Proteins |
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65 | (16) |
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81 | (12) |
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9 Analysis of Protein Glycation Using Phenylboronate Acrylamide Gel Electrophoresis |
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93 | (18) |
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10 Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins |
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111 | (8) |
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11 Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis |
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119 | (16) |
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12 A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples |
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135 | (12) |
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13 Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies |
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147 | (26) |
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14 Microchip Electrophoresis, with Respect to "Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheimer's Disease" |
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173 | (12) |
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15 A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols |
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185 | (12) |
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16 Peptide Fractionation by SDS-Free Polyacrylamide Gel Electrophoresis for Proteomic Analysis via DF-PAGE |
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197 | (8) |
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17 Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass |
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205 | (10) |
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18 SDS-PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting |
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215 | (20) |
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19 Two-Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing |
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235 | (12) |
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20 Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids |
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247 | (12) |
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21 Native Flatbed Isoelectric Focusing for Determining Antibody Clonotype Distribution |
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259 | (8) |
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22 Two-Dimensional Gel Electrophoresis: Glass Tube-Based IEF Followed by SDS-PAGE |
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267 | (8) |
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23 One-Dimensional and Two-Dimensional Immobilized Metal Affinity Electrophoresis |
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275 | (12) |
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24 Two-Dimensional Difference Gel Electrophoresis |
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287 | (18) |
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25 Protein Identification on Archived 2-D Gels |
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305 | (4) |
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26 Diagonal Electrophoresis for the Detection of Protein Disulfides |
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309 | (8) |
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27 Two-Dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes |
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317 | (22) |
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28 Immunoelectrophoresis: A Method with Many Faces |
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339 | (22) |
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29 Cell Surface Protein Biotinylation for SDS-PAGE Analysis |
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361 | (12) |
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30 A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization |
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373 | (12) |
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31 Gel-Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane Proteome |
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385 | (8) |
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32 Interactions of Hemoglobin in Live Red Blood Cells Measured by the Electrophoresis Release Test |
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393 | (10) |
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33 Extraction of Proteins from Gels: A Brief Review |
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403 | (4) |
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34 A New Algorithm for the Simulation of SDS 2D-PAGE Datasets |
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407 | (20) |
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35 Isolation of Proteins from Polyacrylamide Gels |
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427 | (6) |
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433 | (4) |
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37 Long-Term, Buffer-Less, Wet Gel Storage in Non-sealed Polyethylene Bags |
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437 | (8) |
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38 A Brief Review of Other Notable Electrophoretic Methods |
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445 | (6) |
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39 Protein Stains and Applications |
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451 | (14) |
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Natarajan Balasubramaniyan |
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40 Coomassie-Brilliant Blue Staining of Polyacrylamide Gels |
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465 | (6) |
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41 Accelerated Coomassie Blue Staining and Destaining of SDS-PAGE Gels with Application of Heat |
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471 | (10) |
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42 Silver Staining Techniques of Polyacrylamide Gels |
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481 | (6) |
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43 Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis |
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487 | (10) |
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44 Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry |
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497 | (14) |
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45 TEMED-Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots After PAGE |
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511 | (10) |
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46 Microwave-Assisted Protein Staining, Destaining, and In-Gel/In-Solution Digestion of Proteins |
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521 | (12) |
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47 Electrophoretic Separation and Detection of Metalloproteins by X-Ray Fluorescence Mapping |
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533 | (10) |
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48 Fluorescent Staining of Gels |
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543 | (8) |
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49 A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta During Western Blot Analysis |
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551 | (10) |
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50 Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain |
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561 | (6) |
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51 Spicy SDS-PAGE Gels: Curcumin/Turmeric as an Environment-Friendly Protein Stain |
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567 | (12) |
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52 In-Gel Staining of Proteins in Native Polyacrylamide Gel Electrophoresis Using Meso-Tetrakis(4-Sulfonatophenyl) Porphyrin |
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579 | (6) |
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53 Rapid Detection of Proteins in Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black |
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585 | (6) |
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54 Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gel |
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591 | (16) |
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Mircea Alexandru Mateescu |
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55 Coomassie Brilliant Blue Removal/Disposal from Gel Destain and Used Gel Stain in an Environment-Friendly Manner |
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607 | (10) |
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56 A Brief Review of Other Notable Protein Detection Methods on Acrylamide Gels |
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617 | (4) |
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57 Ultrasensitive Protein Detection and Imaging: Comparison of Lumitein™, ProtcoSilver™, SYPRO Ruby, and Coomassic® Brilliant Blue Gel Stains |
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621 | (12) |
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58 Common Artifacts and Mistakes Made in Electrophorcsis |
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633 | (8) |
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| Index |
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641 | |
Daugiau apie elektronines knygas